埃及人的副氧合酶-1(Q192R)基因多态性与心导管术中冠状动脉痉挛的关系。

IF 2.5 4区 医学 Q3 ENDOCRINOLOGY & METABOLISM
Tarek A Abdelaziz, Noha M Mesbah, Dina M Abo-Elmatty, Farah O El-Sabbagh
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引用次数: 0

摘要

背景:冠状动脉痉挛是心肌梗死的病因之一:冠状动脉痉挛是心肌梗死的病因之一。氧化应激与冠状动脉痉挛(CAS)的发病机制有关。副氧合酶-1(PON1)是一种与高密度脂蛋白结合的抗氧化酶,可保护低密度脂蛋白免受氧化修饰。与氧化应激相关的遗传因素和副氧合酶1基因的某些多态性可能会影响CAS的发病机制。我们旨在研究 PON1 基因多态性及其酶活性与心导管检查中冠状动脉痉挛之间的关系:研究对象为 150 名接受择期冠状动脉造影术的患者。通过聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)对受试者的 PON1 基因 Q192R 多态性(rs662)进行基因分型,并通过酶联免疫吸附试验对 PON1 活性进行定量分析。结果显示,携带 RR 基因型和 R 等位基因的受试者发生冠状动脉痉挛的几率明显更高(OR=4.2,2.03,P< 0.006,P˂0.02)。此外,CAS 组的血清 PON1 水平明显下降(P˂0.001)。PON1 Q192R多态性的RR基因型、Tc、LDLc、TG、导管尺寸和血清中的paroxonase-1水平是冠状动脉痉挛的独立预测因子:我们得出结论,埃及人的 PON1(rs662)基因多态性与心导管检查中的 CAS 有关。PON1-192R等位基因和较低的血清酶浓度可能在冠状动脉痉挛中起着重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association of paraoxonase-1 (Q192R) gene polymorphism with coronary artery spasm during cardiac catheterisation in Egyptians.

Background: Coronary artery spasm is among the etiology of myocardial infarction. Oxidative stress is involved in the pathogenesis of coronary artery spasm (CAS). Paraoxonase-1 (PON1) is an HDL-bound antioxidant enzyme that protects LDL from oxidative modification. Oxidative-stress-related genetic factors and certain polymorphisms in the paraoxonase 1 gene might influence the pathogenesis of CAS. We aimed to investigate the association between PON1 gene polymorphism and its enzymatic activity and coronary artery spasm during cardiac catheterization.

Methods and results: The study population was 150 patients who underwent elective coronary angiography. Subjects were genotyped to the Q192R polymorphism (rs662) on the PON1 gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and PON1 activity was quantitatively analyzed by enzyme linked immunosorbent assay. Results showed that the subjects carrying the RR genotype and R allele were significantly more likely to develop coronary artery spasm (OR=4.2, 2.03, P< 0.006, P˂0.02, respectively). Moreover, serum PON1 levels were significantly decreased (P˂0.001) in the CAS group. RR genotype of PON1 Q192R polymorphism, Tc, LDLc, TG, catheter size, and paroxonase-1 serum level are independent predictors of coronary spasm.

Conclusion: We conclude that the PON1 (rs662) gene polymorphism is associated with CAS during cardiac catheterization in Egyptians. The PON1-192R allele and lower serum enzyme concentration may play an important role in coronary spasm.

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来源期刊
Archives of Physiology and Biochemistry
Archives of Physiology and Biochemistry ENDOCRINOLOGY & METABOLISM-PHYSIOLOGY
CiteScore
6.90
自引率
3.30%
发文量
21
期刊介绍: Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders. The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications. Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics: -Dysregulation of hormone receptors and signal transduction -Contribution of gene variants and gene regulatory processes -Impairment of intermediary metabolism at the cellular level -Secretion and metabolism of peptides and other factors that mediate cellular crosstalk -Therapeutic strategies for managing metabolic diseases Special issues dedicated to topics in the field will be published regularly.
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