Souleymane Mahamane Iro, Adamou Lagare, Abdoul-Aziz Maiga, Zara Nouhou, Haladou Gagara, Hadiza Ousmane, Abdoul-Nasser Hassoumi Sanda, Halima Zamanka, Soumana Amadou, Fouta Boubakar, Ibrahima Issa Arzika, Laminou Ibrahim Maman
{"title":"[首次在尼日尔塔胡阿的喙库蚊中发现裂谷热病毒]。","authors":"Souleymane Mahamane Iro, Adamou Lagare, Abdoul-Aziz Maiga, Zara Nouhou, Haladou Gagara, Hadiza Ousmane, Abdoul-Nasser Hassoumi Sanda, Halima Zamanka, Soumana Amadou, Fouta Boubakar, Ibrahima Issa Arzika, Laminou Ibrahim Maman","doi":"10.48327/mtsi.v4i2.2024.512","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The Rift Valley Fever (RVF) is an arbovirus disease responsible of regular epizootics and epidemics in sub-Saharan Africa and Arabian Peninsula. In 2016, Niger experienced its first outbreak of RVF in Tahoua region, which resulted in high consequences in animal and human health. The aim of this study was to investigate on the RVFV circulation among potential vectors of the disease.</p><p><strong>Methods: </strong>This was a cross-sectional survey carried out in Tahoua and Agadez regions in August 2021. Adult mosquitoes were collected by using the morning spray in human dwellings and the CDC light trap methods. After morphological identification, viral RNA was extracted. The RNA was extracted by using QIAamp Viral RNA Mini Kit (Qiagen). The RVFV detection was performed by using the qRT-PCR method.</p><p><strong>Results: </strong>A total of 2487 insects (1978 mosquitoes, 509 sandflies and 251 biting midges) were identified and divided into three families (Culicidae, Psychodidae and Ceratopogonidae). The Culicidae family composed of the <i>Culex</i> genus being the most abundant with a predominance of <i>Cx.pipiens</i> (31.88%; n = 793) followed by <i>Mansonia sp</i> (21.51%; n = 535), <i>Anophelesgambiae</i> s.l. (8.44%; n = 210), <i>An. pharoensis</i> (0.72%; n = 18), <i>An. rufipes</i> (0.48%; n = 12), <i>Cx. quinquefasciatus</i> (6.39%; n = 159), the Psychodidae with sandflies (20.46%; n = 509), and the Ceratopogonidae with <i>Culicoides</i> genus (10.09%; n = 251). The qRT-PCR carried out on a sample of mosquitoes (N = 96) highlighted that one individual of <i>Cx.pipiens</i> was found positive to RVFV. This specimen was from Tassara locality (Tahoua) and collected by CDC Light Trap method.</p><p><strong>Conclusion: </strong>This study reveals for the first time the circulation of RVFV among <i>Cx.pipiens</i> in Niger and highlights the possible vectorial role of this vector in the disease transmission. Further investigations should be carried out to identify the biological and ecological determinants that support the maintenance of the virus in this area in order to guide control interventions.</p>","PeriodicalId":101416,"journal":{"name":"Medecine tropicale et sante internationale","volume":"4 2","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11292430/pdf/","citationCount":"0","resultStr":"{\"title\":\"[First detection of Rift Valley Fever Virus among <i>Culex pipiens</i> in Tahoua, Niger].\",\"authors\":\"Souleymane Mahamane Iro, Adamou Lagare, Abdoul-Aziz Maiga, Zara Nouhou, Haladou Gagara, Hadiza Ousmane, Abdoul-Nasser Hassoumi Sanda, Halima Zamanka, Soumana Amadou, Fouta Boubakar, Ibrahima Issa Arzika, Laminou Ibrahim Maman\",\"doi\":\"10.48327/mtsi.v4i2.2024.512\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The Rift Valley Fever (RVF) is an arbovirus disease responsible of regular epizootics and epidemics in sub-Saharan Africa and Arabian Peninsula. In 2016, Niger experienced its first outbreak of RVF in Tahoua region, which resulted in high consequences in animal and human health. The aim of this study was to investigate on the RVFV circulation among potential vectors of the disease.</p><p><strong>Methods: </strong>This was a cross-sectional survey carried out in Tahoua and Agadez regions in August 2021. Adult mosquitoes were collected by using the morning spray in human dwellings and the CDC light trap methods. After morphological identification, viral RNA was extracted. The RNA was extracted by using QIAamp Viral RNA Mini Kit (Qiagen). The RVFV detection was performed by using the qRT-PCR method.</p><p><strong>Results: </strong>A total of 2487 insects (1978 mosquitoes, 509 sandflies and 251 biting midges) were identified and divided into three families (Culicidae, Psychodidae and Ceratopogonidae). The Culicidae family composed of the <i>Culex</i> genus being the most abundant with a predominance of <i>Cx.pipiens</i> (31.88%; n = 793) followed by <i>Mansonia sp</i> (21.51%; n = 535), <i>Anophelesgambiae</i> s.l. (8.44%; n = 210), <i>An. pharoensis</i> (0.72%; n = 18), <i>An. rufipes</i> (0.48%; n = 12), <i>Cx. quinquefasciatus</i> (6.39%; n = 159), the Psychodidae with sandflies (20.46%; n = 509), and the Ceratopogonidae with <i>Culicoides</i> genus (10.09%; n = 251). The qRT-PCR carried out on a sample of mosquitoes (N = 96) highlighted that one individual of <i>Cx.pipiens</i> was found positive to RVFV. This specimen was from Tassara locality (Tahoua) and collected by CDC Light Trap method.</p><p><strong>Conclusion: </strong>This study reveals for the first time the circulation of RVFV among <i>Cx.pipiens</i> in Niger and highlights the possible vectorial role of this vector in the disease transmission. Further investigations should be carried out to identify the biological and ecological determinants that support the maintenance of the virus in this area in order to guide control interventions.</p>\",\"PeriodicalId\":101416,\"journal\":{\"name\":\"Medecine tropicale et sante internationale\",\"volume\":\"4 2\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11292430/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Medecine tropicale et sante internationale\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.48327/mtsi.v4i2.2024.512\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/6/30 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medecine tropicale et sante internationale","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.48327/mtsi.v4i2.2024.512","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/30 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
[First detection of Rift Valley Fever Virus among Culex pipiens in Tahoua, Niger].
Background: The Rift Valley Fever (RVF) is an arbovirus disease responsible of regular epizootics and epidemics in sub-Saharan Africa and Arabian Peninsula. In 2016, Niger experienced its first outbreak of RVF in Tahoua region, which resulted in high consequences in animal and human health. The aim of this study was to investigate on the RVFV circulation among potential vectors of the disease.
Methods: This was a cross-sectional survey carried out in Tahoua and Agadez regions in August 2021. Adult mosquitoes were collected by using the morning spray in human dwellings and the CDC light trap methods. After morphological identification, viral RNA was extracted. The RNA was extracted by using QIAamp Viral RNA Mini Kit (Qiagen). The RVFV detection was performed by using the qRT-PCR method.
Results: A total of 2487 insects (1978 mosquitoes, 509 sandflies and 251 biting midges) were identified and divided into three families (Culicidae, Psychodidae and Ceratopogonidae). The Culicidae family composed of the Culex genus being the most abundant with a predominance of Cx.pipiens (31.88%; n = 793) followed by Mansonia sp (21.51%; n = 535), Anophelesgambiae s.l. (8.44%; n = 210), An. pharoensis (0.72%; n = 18), An. rufipes (0.48%; n = 12), Cx. quinquefasciatus (6.39%; n = 159), the Psychodidae with sandflies (20.46%; n = 509), and the Ceratopogonidae with Culicoides genus (10.09%; n = 251). The qRT-PCR carried out on a sample of mosquitoes (N = 96) highlighted that one individual of Cx.pipiens was found positive to RVFV. This specimen was from Tassara locality (Tahoua) and collected by CDC Light Trap method.
Conclusion: This study reveals for the first time the circulation of RVFV among Cx.pipiens in Niger and highlights the possible vectorial role of this vector in the disease transmission. Further investigations should be carried out to identify the biological and ecological determinants that support the maintenance of the virus in this area in order to guide control interventions.
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