黄酮通过调节PI3K/Nrf2和FoxO1/NLRP3炎性体减轻伽马射线照射大鼠尼古丁诱发的肺损伤

IF 3.5 3区 医学
Nora A Elsayed, Mohammed A Marzouk, Fatma Sm Moawed, Esraa Sa Ahmed, Omayma Ar Abo-Zaid
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引用次数: 0

摘要

长期暴露于不同的职业或环境毒物会引发氧化应激和炎症反应介导的肺损伤。本研究旨在探讨黄酮对尼古丁(NIC)中毒和辐射(IR)暴露大鼠肺损伤的影响和保护作用。研究将 40 只大鼠分为四组:第一组为对照组;第二组为黄酮组;给大鼠服用黄酮(25 毫克/千克/天);第三组为尼古丁+IR 组;给大鼠腹腔注射尼古丁(1 毫克/千克/天)并暴露于γ-IR(3.5 Gy,每周一次,持续 2 周);第四组为尼古丁+IR+黄酮组;给大鼠注射尼古丁、暴露于 IR 并服用黄酮。对肺组织的氧化还原状态参数和组织病理学变化进行了评估。测量了肺组织中核因子卡巴B(NF-κB)、叉头盒O-类1(FoxO1)和核苷酸结合域(NOD)样受体含吡啶域-3(NLRP3)基因的表达。此外,还使用酶联免疫吸附试剂盒检测了核因子(红细胞衍生 2)样 2(Nrf2)和磷脂酰肌醇三激酶(PI3K)。我们的数据首次证明,黄酮可通过激活 PI3K/Nrf2 来减轻氧化还原状态的破坏并加强抗氧化防御机制,从而保护肺部免受 NIC/IR 相关细胞毒性的伤害。此外,黄酮还能通过抑制炎症信号通路 FOXO1/NF-κB/NLRP3- Inflammasome,缓解肺部炎症。总之,研究结果表明,黄酮可通过调节PI3K/Nrf2和FoxO1/NLRP3炎症体,有效减轻NIC和IR诱发的肺损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Flavone attenuates nicotine-induced lung injury in rats exposed to gamma radiation via modulating PI3K/Nrf2 and FoxO1/NLRP3 inflammasome.

Prolonged exposure to different occupational or environmental toxicants triggered oxidative stress and inflammatory reactions mediated lung damage. This study was designed to explore the influence and protective impact of flavone on lung injury in rats intoxicated with nicotine (NIC) and exposed to radiation (IR). Forty rats were divided into four groups; group I control, group II flavone; rats were administered with flavone (25 mg/kg/day), group III NIC + IR; rats were injected intraperitoneally with NIC (1 mg/kg/day) and exposed to γ-IR (3.5 Gy once/week for 2 weeks) while group IV NIC + IR + flavone; rats were injected with NIC, exposed to IR and administered with flavone. Redox status parameters and histopathological changes in lung tissue were evaluated. Nuclear factor-kappa B (NF-κB), forkhead box O-class1 (FoxO1) and nucleotide-binding domain- (NOD-) like receptor pyrin domain-containing-3 (NLRP3) gene expression were measured in lung tissues. Moreover, nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and phosphatidylinositol three kinase (PI3K) were measured using ELISA kits. Our data demonstrates, for the first time, that flavone protects the lung from NIC/IR-associated cytotoxicity, by attenuating the disrupted redox status and aggravating the antioxidant defence mechanism via activation of the PI3K/Nrf2. Moreover, flavone alleviates pulmonary inflammation by inhibiting the inflammatory signaling pathway FOXO1/NF-κB/NLRP3- Inflammasome. Collectively, the obtained results exhibited a notable efficiency of flavone in alleviating lung injury induced by NIC and IR via modulating PI3K/Nrf2 and FoxO1/NLRP3 Inflammasome.

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来源期刊
International Journal of Immunopathology and Pharmacology
International Journal of Immunopathology and Pharmacology Immunology and Microbiology-Immunology
自引率
0.00%
发文量
88
期刊介绍: International Journal of Immunopathology and Pharmacology is an Open Access peer-reviewed journal publishing original papers describing research in the fields of immunology, pathology and pharmacology. The intention is that the journal should reflect both the experimental and clinical aspects of immunology as well as advances in the understanding of the pathology and pharmacology of the immune system.
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