四氢异喹啉通过细胞外囊泡介导骨髓瘤细胞与 HUVEC 的相互作用,减少血管生成。

IF 2.8 4区 医学 Q2 ONCOLOGY
Ahmad Kooshari, Fahimeh Shahriyary, Minoo Shahidi, Mahshid Vafajoo, Mohammad Reza Amirzargar
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引用次数: 0

摘要

多发性骨髓瘤(MM)是一种由 B 细胞衍生的浆细胞不受控制地扩增而导致的肿瘤性疾病。血管生成在 MM 发展过程中的重要性也已得到证实。细胞外小泡(EVs)在相邻细胞间的相互作用(如血管生成)中具有重要功能。这项体外研究的目的是检测 MM-EVs 经四氢异喹啉(THIQ)处理后对内皮细胞(ECs)的转染和血管生成效应,四氢异喹啉是异喹啉的一种生物活性有机化合物衍生物。用四氢异喹啉处理多发性骨髓瘤细胞(U266)后,在共培养模型中收获 MM-EVs 并将其转移到人脐静脉内皮细胞(HUVEC)上。通过流式细胞术追踪了EVs的迁移。相应地,通过 RT-PCR 和 ELISA 方法测量了 U266 细胞和 HUVEC 中血管生成基因和/或蛋白质的表达。同样,用管形成法和划痕伤口愈合法观察和评估了经 THIQ 处理的 MM-EV 处理的 HUVEC 的增殖和迁移情况。令人惊讶的是,经 THIQ 处理的 MM-EVs 在 HUVEC 内化后,CD34、VEGFR2 和 IL-6 在 mRNA 和/或蛋白水平上的表达量均有所下降,这表明 MM-EVs 具有抗血管生成的作用。最后,与对照MM-EV相比,管形成和划痕伤口愈合实验表明,THIQ处理的MM-EV抑制了HUVEC细胞的增殖和迁移。由 THIQ 处理过的骨髓瘤细胞(U266)衍生的 MM-EVs 抑制了 HUVEC 的血管生成。这一现象是由 HUVECs 内化的 THIQ 处理过的 MM-EVs 协调的,最终减少了血管生成因子,抑制了管形成和划痕伤口愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Tetrahydroisoquinoline reduces angiogenesis by interacting myeloma cells with HUVECs mediated by extracellular vesicles.

Tetrahydroisoquinoline reduces angiogenesis by interacting myeloma cells with HUVECs mediated by extracellular vesicles.

Multiple myeloma (MM) is a neoplastic condition resulting from the uncontrolled expansion of B-cell-derived plasma cells. The importance of angiogenesis in MM development has also been demonstrated. Extracellular vesicles (EVs) have vital functions in interactions between neighboring cells, such as angiogenesis. The objective of this in vitro study was to examine the transfection and angiogenesis effects of MM-EVs on endothelial cells (ECs) upon treatment with Tetrahydroisoquinoline (THIQ) as a bioactive organic compound derivative from isoquinoline. Following treatment of multiple myeloma cells (U266) with THIQ, MM-EVs were harvested and transmigrated to human umbilical vein endothelial cells (HUVEC) in a co-culture model. EVs transmigration was traced by flow cytometry. Correspondingly, the expression of angiogenic genes and/or proteins in U266 cells and HUVECs was measured by RT-PCR and ELISA methods. Likewise, the proliferation and migration of HUVECs treated with THIQ-treated MM-EVs were visualized and estimated by performing both tube formation and scratch wound healing methods. Surprisingly, the anti-angiogenic effect of THIQ-treated MM-EVs was evident by the decreased expression of CD34, VEGFR2, and IL-6 at the mRNA and/or protein levels after internalization of MM-EVs in HUVEC. Finally, tube formation and scratch wound healing experiments showed inhibition of HUVEC cell proliferation and migration by THIQ-treated MM-EVs compared to control MM-EVs. MM-EVs derived from THIQ-treated myeloma cells (U266) inhibited angiogenesis in HUVECs. This phenomenon is coordinated by the internalized THIQ-treated MM-EVs in HUVECs, and ultimately the reduction of angiogenic factors and inhibition of tube formation and scratch wound healing.

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来源期刊
Medical Oncology
Medical Oncology 医学-肿瘤学
CiteScore
4.20
自引率
2.90%
发文量
259
审稿时长
1.4 months
期刊介绍: Medical Oncology (MO) communicates the results of clinical and experimental research in oncology and hematology, particularly experimental therapeutics within the fields of immunotherapy and chemotherapy. It also provides state-of-the-art reviews on clinical and experimental therapies. Topics covered include immunobiology, pathogenesis, and treatment of malignant tumors.
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