南非多尔帕羊 Toll 样受体 4 基因第 2 外显子的遗传变异。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-06-08 eCollection Date: 2024-06-01 DOI:10.5455/javar.2024.k777

Lebelo Selala, Teedzai Chitura, Vusi Mbazima, Louis Tyasi

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引用次数: 0

摘要

研究目的本研究旨在确定南非多尔巴绵羊 Toll 样受体 4 (TLR4) 外显子 2 的序列变异：从 50 只（n = 50）年龄在 3 至 4 岁之间的南非多尔巴绵羊身上采集血液样本。对脱氧核糖核酸（DNA）进行提取、扩增和 TLR4 基因测序。DNA 测序用于确定南非多尔巴绵羊 TLR4 基因的序列变异：结果表明，在 TLR4 基因第 2 外显子位置 T2249C 发现了一个同义单核苷酸多态性（SNP）。通过该 SNP 发现了两种基因型（TT 和 TC）。显性基因型为 TT（0.60）高于 TC（0.40），显性等位基因为 T（0.80）高于 C（0.20）。结果还表明，所用人群处于哈德-温伯格平衡状态。多态性遗传分析结果表明，南非多尔巴绵羊 TLR4 外显子 2 的序列变异属于中度多态性：南非多尔巴绵羊 TLR4 基因外显子 2 的 SNP（T>C）位于 2249 bp 位置，有两种基因型（TT 和 TC）。

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Genetic variations of Toll-like receptor 4 gene in exon 2 of South African Dorper sheep.

Objective: The study was conducted to identify the sequence variation of Toll-like receptor 4 (TLR4) in exon 2 of South African Dorper sheep.

Materials and methods: Blood samples were collected from fifty (n = 50) South African Dorper sheep aged between 3 and 4 years. The Deoxyribonucleic acid (DNA) was extracted, amplified, and sequenced for the TLR4 gene. DNA sequencing was used to identify the sequence variations of the TLR4 gene in South African Dorper sheep.

Results: The results showed that one synonymous single nucleotide polymorphism (SNP) of the TLR4 gene in exon 2 position T2249C was identified. Two genotypes (TT and TC) were discovered from the identified SNP. The dominant genotype was TT (0.60) over TC (0.40), with the dominant allele T (0.80) over C (0.20). The results also indicated that the used population was in the Hady-Weinberg Equilibrium. Polymorphism genetic analysis findings suggest that the identified sequence variation of TLR4 in exon 2 of South African Dorper sheep was moderate polymorphism.

Conclusion: TLR4 gene at exon 2 of South African Dorper sheep had the SNP (T>C) at position 2249 bp with two genotypes (TT and TC).

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.