随着高分辨率结构的积累而揭示的 DNA 模拟基准。

IF 4.9 Q1 BIOPHYSICS
Biophysical reviews Pub Date : 2024-06-18 eCollection Date: 2024-06-01 DOI:10.1007/s12551-024-01198-2
Wilma K Olson, Robert T Young, Luke Czapla
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引用次数: 0

摘要

DNA 所承载的不仅仅是驱动生命系统基本功能的一系列生化指令。碱基对序列包含了大量的结构和能量信号,这些信号决定了线状长分子的运动程度,以及它如何对参与其加工和包装的蛋白质和其他分子做出反应。高分辨率蛋白质-DNA 晶体结构中连续碱基对的排列为双螺旋 DNA 的原子级模拟提供了有用的基准,也为解释特定 DNA 序列的特性提供了潜在的有用信息。目前可用的一组结构有足够多的实例来描述 DNA 碱基对步骤在其近邻(即四聚体)范围内的构象偏好,并揭示了某些近邻对局部链特性的惊人影响。与 DNA 接触的蛋白质呈现出各种微环境,这些微环境能够感知和/或诱导观察到的空间形式。不同蛋白质-DNA 复合物中氨基酸原子的累积形成了双螺旋周围的结合云,这种结合云具有微妙的序列依赖性特征。虽然每种蛋白质与 DNA 之间的微环境都非常独特,但在广泛的结构集合中,DNA 近距离内氨基酸原子的整体组成却相当一致。DNA 周围不同类型蛋白质原子的堆积为改进核酸力场提供了新的信息,也为探索 DNA 在溶液中的特性提供了新的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
DNA simulation benchmarks revealed with the accumulation of high-resolution structures.

DNA carries more than the list of biochemical instructions that drive the basic functions of living systems. The sequence of base pairs includes a multitude of structural and energetic signals that determine the degree to which the long, threadlike molecule moves and how it responds to proteins and other molecules involved in its processing and packaging. The arrangements of successive base pairs in high-resolution protein-DNA crystal structures provide useful benchmarks for atomic-level simulations of double-helical DNA as well as information potentially useful in interpreting the properties of specific DNA sequences. The set of currently available structures has enough examples to characterize the conformational preferences of the DNA base-pair steps within the context of their immediate neighbors, i.e., in the context of tetramers, and reveals surprising effects of certain neighbors on local chain properties. The proteins in contact with DNA present various microenvironments that sense and/or induce the observed spatial forms. The cumulative buildup of amino-acid atoms in different protein-DNA complexes produces a binding cloud around the double helix with subtle sequence-dependent features. While the microenvironment presented by each protein to DNA is highly unique, the overall composition of amino-acid atoms within close range of DNA in a broad collection of structures is fairly uniform. The buildup of protein atoms of different types around the DNA provides new information for the improvement of nucleic acid force fields and fresh ideas for the exploration of the properties of DNA in solution.

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来源期刊
Biophysical reviews
Biophysical reviews Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
8.90
自引率
0.00%
发文量
93
期刊介绍: Biophysical Reviews aims to publish critical and timely reviews from key figures in the field of biophysics. The bulk of the reviews that are currently published are from invited authors, but the journal is also open for non-solicited reviews. Interested authors are encouraged to discuss the possibility of contributing a review with the Editor-in-Chief prior to submission. Through publishing reviews on biophysics, the editors of the journal hope to illustrate the great power and potential of physical techniques in the biological sciences, they aim to stimulate the discussion and promote further research and would like to educate and enthuse basic researcher scientists and students of biophysics. Biophysical Reviews covers the entire field of biophysics, generally defined as the science of describing and defining biological phenomenon using the concepts and the techniques of physics. This includes but is not limited by such areas as: - Bioinformatics - Biophysical methods and instrumentation - Medical biophysics - Biosystems - Cell biophysics and organization - Macromolecules: dynamics, structures and interactions - Single molecule biophysics - Membrane biophysics, channels and transportation
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