基于微流体的纳米粒子制造工艺优化计算辅助方法。

IF 3 2区 医学 Q3 ENGINEERING, BIOMEDICAL
Marco Bellotti, Enrica Chiesa, Bice Conti, Ida Genta, Michele Conti, Ferdinando Auricchio, Alessandro Caimi
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引用次数: 0

摘要

在过去几年中,由于生产效率高、可变性低、生产参数可控性强,纳米粒子(NPs)的微流体生产正在成为传统工业方法(如纳米沉淀法、盐析法和乳化扩散法)的一种有前途的替代方法。然而,新配方的开发和生产工艺向微流控平台的转变需要大量昂贵而耗时的实验来调整配方,以获得具有特定形态和功能特征的 NPs。在这项工作中,我们开发了一个计算流体动力学管道,并通过特别实验策略进行了验证,以重现溶剂和反溶剂(即分别为乙腈和 TRIS-HCl)之间的混合。此外,除了能够描述微流控芯片混合性能的经典变量外,还描述了新的变量,以评估 NPs 形成区域以及沉淀区域的振幅随不同水力条件的变化。事实证明,数值方法能够捕捉到纳米沉淀区域因流速比增加而逐渐缩小的现象;与此同时,通过实验生产,在相同的流体动力学条件下观察到了 NPs 尺寸异质性的逐渐增加。因此,数值和实验证据的初步比较证明了计算策略在优化 NPs 制造过程中的有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Computational-Aided Approach for the Optimization of Microfluidic-Based Nanoparticles Manufacturing Process

Computational-Aided Approach for the Optimization of Microfluidic-Based Nanoparticles Manufacturing Process

In the last few years, the microfluidic production of nanoparticles (NPs) is becoming a promising alternative to conventional industrial approaches (e.g., nanoprecipitation, salting out, and emulsification-diffusion) thanks to the production efficiency, low variability, and high controllability of the production parameters. Nevertheless, the development of new formulations and the switching of the production process toward microfluidic platforms requires expensive and time-consuming number of experiments for the tuning of the formulation to obtain NPs with specific morphological and functional characteristics. In this work, we developed a computational fluid dynamic pipeline, validated through an ad hoc experimental strategy, to reproduce the mixing between the solvent and anti-solvent (i.e., acetonitrile and TRIS–HCl, respectively). Moreover, beyond the classical variables able to describe the mixing performances of the microfluidic chip, novel variables were described in order to assess the region of the NPs formation and the changing of the amplitude of the precipitation region according to different hydraulic conditions. The numerical approach proved to be able to capture a progressive reduction of the nanoprecipitation region due to an increment of the flow rate ratio; in parallel, through the experimental production, a progressive increment of the NPs size heterogeneity was observed with the same fluid dynamic conditions. Hence, the preliminary comparison between numerical and experimental evidence proved the effectiveness of the computational strategy to optimize the NPs manufacturing process.

Graphical Abstract

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来源期刊
Annals of Biomedical Engineering
Annals of Biomedical Engineering 工程技术-工程:生物医学
CiteScore
7.50
自引率
15.80%
发文量
212
审稿时长
3 months
期刊介绍: Annals of Biomedical Engineering is an official journal of the Biomedical Engineering Society, publishing original articles in the major fields of bioengineering and biomedical engineering. The Annals is an interdisciplinary and international journal with the aim to highlight integrated approaches to the solutions of biological and biomedical problems.
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