在沙特阿拉伯吉达实地采集的埃及伊蚊中检测沃尔巴克氏体

IF 1.2 4区农林科学 Q3 ENTOMOLOGY

Entomological Research Pub Date : 2024-08-02 DOI:10.1111/1748-5967.12759

Mahmoud A. Emara, Nuha Mustafa Altilmisani, Faisal Albishri, Imtinan Akram Khan, Salah Mubark Elkhalifa, Talha A. Al-Dubai, Esam Omar Al-Wesabi

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引用次数: 0

摘要

最近有报道称，埃及伊蚊体内存在沃尔巴克氏体。我们的研究通过使用两种沃尔巴克氏体特异性分子标记筛选野生成蚊，检测埃及伊蚊是否感染了沃尔巴克氏体。从 2022 年 4 月到 2022 年 10 月，我们在沙特阿拉伯吉达共采集了 444 只埃及成蚊。在优化的聚合酶链式反应（PCR）条件下，对每个样本进行处理，并使用选定的标记--沃尔巴克氏体特异性 16S rDNA 和沃尔巴克氏体表面蛋白基因（wsp）--筛查是否存在沃尔巴克氏体，然后进行测序。利用 wsp 和 16S rDNA 标记分别确定了 39（8.78%）和 48（10.81%）个蚊子样本感染了沃尔巴克氏体。通过使用两种沃尔巴克氏体特异性分子标记，我们的研究证明了埃及蚁个体样本中存在沃尔巴克氏体。我们的研究结果显示沃尔巴克氏体的感染率较低，并推断检测到的菌株属于超群 B。

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Detection of Wolbachia in field-collected Aedes aegypti mosquitoes from Jeddah, Saudi Arabia

Recent reports have disclosed the occurrence of Wolbachia in Aedes aegypti. Our study detected Wolbachia infection in Ae. aegypti by screening wild adult mosquitoes using two Wolbachia-specific molecular markers. Overall, 444 adult Ae. aegypti mosquitoes were collected from April 2022 to October 2022 in Jeddah, Saudi Arabia. Each individual sample was processed and screened for the presence of Wolbachia using selected markers, the Wolbachia-specific 16S rDNA and the Wolbachia surface protein gene (wsp), under optimized polymerase chain reaction (PCR) conditions, and sequenced. In total, 39 (8.78%) and 48 (10.81%) individual mosquito samples were determined to be infected with Wolbachia using the wsp and 16S rDNA markers, respectively. By utilizing two Wolbachia-specific molecular markers, our study demonstrated the presence of Wolbachia from individual Ae. aegypti samples. Our results showed a low rate of Wolbachia infection and inferred that the detected strain belongs to supergroup B.

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来源期刊

Entomological Research ENTOMOLOGY-

CiteScore

2.50

自引率

7.70%

发文量

期刊介绍： Entomological Research is the successor of the Korean Journal of Entomology. Published by the Entomological Society of Korea (ESK) since 1970, it is the official English language journal of ESK, and publishes original research articles dealing with any aspect of entomology. Papers in any of the following fields will be considered: -systematics- ecology- physiology- biochemistry- pest control- embryology- genetics- cell and molecular biology- medical entomology- apiculture and sericulture. The Journal publishes research papers and invited reviews.