固定金黄色葡萄球菌和肠炎沙门氏菌生物膜以便进行显微镜检查的方法

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2024-08-03 DOI:10.1007/s10517-024-06174-0

A. E. Grigor’eva, A. V. Tupitsyna, A. V. Bardasheva, E. S. Ryabova, E. I. Ryabchikova

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引用次数: 0

摘要

比较了固定金黄色葡萄球菌和肠炎沙门氏菌生物膜进行光镜和电镜观察的不同方法。多聚甲醛固定法不能在脱水过程中保持生物膜的完整性；伊托-卡诺夫斯基固定法能显示细胞形态，但不能保留基质。钌红与醛类结合可保存基质并使其可视化。本文对不同固定方法下生物膜和悬浮液中金黄色葡萄球菌和肠杆菌细胞的超微结构进行了分析。描述了生物膜基质的超微结构。

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Methods for Fixing Biofilms of Staphylococcus aureus and Salmonella enterica for Microscopic Examination

Different methods for fixing biofilms of Staphylococcus aureus and Salmonella enterica for light and electron microscopy were compared. Paraformaldehyde fixation did not preserve biofilm integrity during dehydration; Ito—Karnovsky fixation revealed cell morphology, but did not preserve the matrix. Ruthenium red combined with aldehydes allowed the matrix to be preserved and visualized. An analysis of the ultrastructure of S. aureus and S. enterica cells in biofilms and suspensions at various fixations is presented. The ultrastructure of the biofilm matrix has been described.

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.