基于离子液体的透明膜耦合人肺上皮芯片在呼吸机械压力下展示 PM0.5 污染效应

IF 8.1 1区 医学 Q1 ENGINEERING, BIOMEDICAL
Bilgesu Kaya, Ozlem Yesil-Celiktas
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引用次数: 0

摘要

在过去几年中,人类接触颗粒物(PM)的可能性越来越大。由于灰尘迁移在天气和气候构成中的作用,人们在大气中发现了不同大小的可吸入颗粒物。作为调节器,肺上皮细胞会协调对局部损伤的先天性反应。在此,我们开发了一种肺上皮芯片平台,该平台由易于成型的聚二甲基硅氧烷层和薄而柔韧的透明离子液体基聚(羟乙基)甲基丙烯酸酯凝胶膜组成。人肺上皮细胞(Calu-3)就是在这层膜上培养形成的。利用基于 Arduino 的伺服电机系统重现了吸气/呼气过程中气液界面的机械应力,该系统从芯片两侧施加单轴拉伸强度,应变为 10%,频率为 0.2 Hz。随后,在静态、动态和动态+机械应力(DMS)条件下,向芯片平台施加平均粒径为 463 nm 的二氧化硅纳米颗粒(PM0.5),证明了环境污染物对肺上皮细胞的影响。通过定量检测肿瘤坏死因子-α,测定了肺上皮细胞的活力和乳酸脱氢酶的释放以及促炎反应,这表明肺上皮细胞发生了改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Ionic liquid-based transparent membrane-coupled human lung epithelium-on-a-chip demonstrating PM0.5 pollution effect under breathing mechanostress

Ionic liquid-based transparent membrane-coupled human lung epithelium-on-a-chip demonstrating PM0.5 pollution effect under breathing mechanostress

The plausibility of human exposure to particulate matter (PM) has witnessed an increase within the last several years. PM of different sizes has been discovered in the atmosphere given the role of dust transport in weather and climate composition. As a regulator, the lung epithelium orchestrates the innate response to local damage. Herein, we developed a lung epithelium-on-a-chip platform consisting of easily moldable polydimethylsiloxane layers along with a thin, flexible, and transparent ionic liquid-based poly(hydroxyethyl) methacrylate gel membrane. The epithelium was formed through the culture of human lung epithelial cells (Calu-3) on this membrane. The mechanical stress at the air–liquid interface during inhalation/exhalation was recapitulated using an Arduino-based servo motor system, which applied a uniaxial tensile strength from the two sides of the chip with 10% strain and a frequency of 0.2 Hz. Subsequently, the administration of silica nanoparticles (PM0.5) with an average size of 463 nm to the on-chip platform under static, dynamic, and dynamic + mechanical stress (DMS) conditions demonstrated the effect of environmental pollutants on lung epithelium. The viability and release of lactate dehydrogenase were determined along with proinflammatory response through the quantification of tumor necrosis factor-α, which indicated alterations in the epithelium.

Graphic abstract

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来源期刊
Bio-Design and Manufacturing
Bio-Design and Manufacturing Materials Science-Materials Science (miscellaneous)
CiteScore
13.30
自引率
7.60%
发文量
148
期刊介绍: Bio-Design and Manufacturing reports new research, new technology and new applications in the field of biomanufacturing, especially 3D bioprinting. Topics of Bio-Design and Manufacturing cover tissue engineering, regenerative medicine, mechanical devices from the perspectives of materials, biology, medicine and mechanical engineering, with a focus on manufacturing science and technology to fulfil the requirement of bio-design.
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