Li Yuan, Chen Yuan, Jiawei Wei, Shue Jin, Yi Zuo, Yubao Li, Xinjie Liang, Jidong Li
{"title":"电纺丝/三维打印一体化多孔支架引导小猎犬口腔组织再生","authors":"Li Yuan, Chen Yuan, Jiawei Wei, Shue Jin, Yi Zuo, Yubao Li, Xinjie Liang, Jidong Li","doi":"10.1007/s42242-024-00311-4","DOIUrl":null,"url":null,"abstract":"<p>The combined use of guided tissue/bone regeneration (GTR/GBR) membranes and bone filling grafts represents a classical therapy for guiding the regeneration and functional reconstruction of oral soft and hard tissues. Nevertheless, due to its displacement and poor mechanical support, bone meal is not suitable for implantation in the case of insufficient cortical bone support and large dimensional defects. The combination of GTR/GBR membrane with a three-dimensional (3D) porous scaffold may offer a resolution for the repair and functional reconstruction of large soft and hard tissue defects. In this study, a novel integrated gradient biodegradable porous scaffold was prepared by bonding a poly(lactic-co-glycolic acid) (PLGA)/fish collagen (FC) electrospun membrane (PFC) to a 3D-printed PLGA/nano-hydroxyapatite (HA) (PHA) scaffold. The consistency of the composition (PLGA) ensured strong interfacial bonding between the upper fibrous membrane and the lower 3D scaffold. In vitro cell experiments showed that the PFC membrane (upper layer) effectively prevented the unwanted migration of L929 cells. Further in vivo investigations with an oral soft and hard tissue defect model in beagles revealed that the integrated scaffold effectively guided the regeneration of defective oral tissues. These results suggest that the designed integrated scaffold has great potential for guiding the regeneration and reconstruction of large oral soft and hard tissues.</p><h3 data-test=\"abstract-sub-heading\">Graphic abstract</h3>\n","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":null,"pages":null},"PeriodicalIF":8.1000,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Electrospinning/3D printing-integrated porous scaffold guides oral tissue regeneration in beagles\",\"authors\":\"Li Yuan, Chen Yuan, Jiawei Wei, Shue Jin, Yi Zuo, Yubao Li, Xinjie Liang, Jidong Li\",\"doi\":\"10.1007/s42242-024-00311-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The combined use of guided tissue/bone regeneration (GTR/GBR) membranes and bone filling grafts represents a classical therapy for guiding the regeneration and functional reconstruction of oral soft and hard tissues. Nevertheless, due to its displacement and poor mechanical support, bone meal is not suitable for implantation in the case of insufficient cortical bone support and large dimensional defects. The combination of GTR/GBR membrane with a three-dimensional (3D) porous scaffold may offer a resolution for the repair and functional reconstruction of large soft and hard tissue defects. In this study, a novel integrated gradient biodegradable porous scaffold was prepared by bonding a poly(lactic-co-glycolic acid) (PLGA)/fish collagen (FC) electrospun membrane (PFC) to a 3D-printed PLGA/nano-hydroxyapatite (HA) (PHA) scaffold. The consistency of the composition (PLGA) ensured strong interfacial bonding between the upper fibrous membrane and the lower 3D scaffold. In vitro cell experiments showed that the PFC membrane (upper layer) effectively prevented the unwanted migration of L929 cells. Further in vivo investigations with an oral soft and hard tissue defect model in beagles revealed that the integrated scaffold effectively guided the regeneration of defective oral tissues. These results suggest that the designed integrated scaffold has great potential for guiding the regeneration and reconstruction of large oral soft and hard tissues.</p><h3 data-test=\\\"abstract-sub-heading\\\">Graphic abstract</h3>\\n\",\"PeriodicalId\":48627,\"journal\":{\"name\":\"Bio-Design and Manufacturing\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":8.1000,\"publicationDate\":\"2024-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bio-Design and Manufacturing\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s42242-024-00311-4\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bio-Design and Manufacturing","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s42242-024-00311-4","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
Electrospinning/3D printing-integrated porous scaffold guides oral tissue regeneration in beagles
The combined use of guided tissue/bone regeneration (GTR/GBR) membranes and bone filling grafts represents a classical therapy for guiding the regeneration and functional reconstruction of oral soft and hard tissues. Nevertheless, due to its displacement and poor mechanical support, bone meal is not suitable for implantation in the case of insufficient cortical bone support and large dimensional defects. The combination of GTR/GBR membrane with a three-dimensional (3D) porous scaffold may offer a resolution for the repair and functional reconstruction of large soft and hard tissue defects. In this study, a novel integrated gradient biodegradable porous scaffold was prepared by bonding a poly(lactic-co-glycolic acid) (PLGA)/fish collagen (FC) electrospun membrane (PFC) to a 3D-printed PLGA/nano-hydroxyapatite (HA) (PHA) scaffold. The consistency of the composition (PLGA) ensured strong interfacial bonding between the upper fibrous membrane and the lower 3D scaffold. In vitro cell experiments showed that the PFC membrane (upper layer) effectively prevented the unwanted migration of L929 cells. Further in vivo investigations with an oral soft and hard tissue defect model in beagles revealed that the integrated scaffold effectively guided the regeneration of defective oral tissues. These results suggest that the designed integrated scaffold has great potential for guiding the regeneration and reconstruction of large oral soft and hard tissues.
期刊介绍:
Bio-Design and Manufacturing reports new research, new technology and new applications in the field of biomanufacturing, especially 3D bioprinting. Topics of Bio-Design and Manufacturing cover tissue engineering, regenerative medicine, mechanical devices from the perspectives of materials, biology, medicine and mechanical engineering, with a focus on manufacturing science and technology to fulfil the requirement of bio-design.