小鼠与甲基汞共同暴露后,膳食蛋白质会影响汞的组织积累,并诱导肝脏 I 期和 II 期酶的表达。

IF 4.8 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ragnhild Marie Mellingen , Josef Daniel Rasinger , Ole Jakob Nøstbakken , Lene Secher Myrmel , Annette Bernhard
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引用次数: 0

摘要

甲基汞(MeHg)是一种无处不在的环境污染物,以其神经毒性作用而闻名。甲基汞可与膳食中的多种营养物质相互作用,影响营养物质的新陈代谢,但甲基汞与膳食蛋白质之间的相互作用尚未得到深入研究。雄性 BALB/c 小鼠的饮食以酪蛋白、鳕鱼或鸡肉为蛋白质来源,并添加或不添加甲基汞(3.5 毫克汞/千克)。喂食酪蛋白和甲基汞的小鼠体内汞含量最高,喂食鳕鱼和甲基汞的小鼠体内汞含量较低,而喂食鸡肉和甲基汞的小鼠体内汞含量最低。对肠道微生物群的评估显示,不同蛋白质来源的微生物群组成存在差异,但甲基汞的引入消除了这种差异。肝脏组织的蛋白质组分析揭示了膳食蛋白质来源对一系列与第一阶段和第二阶段解毒机制相关的酶的影响,表明膳食对甲基汞的代谢和排泄有影响。此外,与蛋氨酸和甘氨酸甜菜碱循环等途径有关的酶在以鸡肉为膳食蛋白质的小鼠体内也出现了上调,而蛋氨酸和甘氨酸甜菜碱循环反过来又会影响谷胱甘肽(一种重要的甲基汞共轭分子)的产生。我们的研究结果表明,膳食蛋白质会影响肝脏酶的表达,而肝脏酶可能会影响甲基汞的代谢和排泄,这突出了在通过膳食接触甲基汞的风险评估中考虑膳食成分的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dietary protein affects tissue accumulation of mercury and induces hepatic Phase I and Phase II enzyme expression after co-exposure with methylmercury in mice

Methylmercury (MeHg) is a ubiquitous environmental contaminant, well known for its neurotoxic effects. MeHg can interact with several nutrients in the diet and affect nutrient metabolism, however the interaction between MeHg and dietary proteins has not been thoroughly investigated. Male BALB/c mice were fed diets based on either casein, cod or chicken as protein sources, which were or were not spiked with MeHg (3.5 mg Hg kg−1). Following 13 weeks of dietary exposure to MeHg, the animals accumulated mercury in a varying degree depending on the diet, where the levels of mercury were highest in the mice fed casein and MeHg, lower in mice fed cod and MeHg, and lowest in mice fed chicken and MeHg in all tissues assessed. Assessment of gut microbiota revealed differences in microbiota composition based on the different protein sources. However, the introduction of MeHg eliminated this difference. Proteomic profiling of liver tissue uncovered the influence of the dietary protein sources on a range of enzymes related to Phase I and Phase II detoxification mechanisms, suggesting an impact of the diet on MeHg metabolism and excretion. Also, enzymes linked to pathways including methionine and glycine betaine cycling, which in turn impact the production of glutathione, an important MeHg conjugation molecule, were up-regulated in mice fed chicken as dietary protein. Our findings indicate that dietary proteins can affect expression of hepatic enzymes that potentially influence MeHg metabolism and excretion, highlighting the relevance of considering the dietary composition in risk assessment of MeHg through dietary exposure.

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来源期刊
Journal of Nutritional Biochemistry
Journal of Nutritional Biochemistry 医学-生化与分子生物学
CiteScore
9.50
自引率
3.60%
发文量
237
审稿时长
68 days
期刊介绍: Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology. Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.
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