长春西汀和乳酸菌可改善大鼠的脂肪肝:脂肪连接素和肠道微生物组的作用。

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ahmed M El-Baz, Ahmed Shata, Nehal A Nouh, Lubna Jamil, Mohamed M Hafez, Sally Negm, Attalla F El-Kott, Mohammed A AlShehri, Eman M Khalaf
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引用次数: 0

摘要

干扰损伤/病原体相关分子模式(DAMPs/PAMPs)的治疗方法已成为治疗非酒精性脂肪肝(NAFLD)等肝脏炎症的有前途的候选疗法。在本研究中,我们考察了磷酸二酯酶-1 抑制剂长春西汀(Vinpo)单独或与乳酸杆菌联合使用对大鼠因 13 周高脂肪饮食(HFD)和糖尿病引起的肝功能异常的治疗效果。结果表明,Vinpo(10 和 20 毫克/公斤/天)剂量依赖性地抑制了高脂饮食引起的血清(谷丙转氨酶、谷草转氨酶)和组织病理学中肝脏损伤参数的升高。这些作用与 Vinpo 改善高频分解诱导的肝纤维化(组织学纤维化评分、羟脯氨酸、TGF-β1)和氧化应激(MDA、NOx)以及恢复肝脏中抗氧化相关参数(GSH、SOD、Nrf-2、HO-1)的潜力是一致的。从机理上讲,Vinpo 可减少肝细胞释放高迁移率基团框(HMGB)1 等 DAMPs,同时降低模式识别受体(包括类收费受体(TLR)4 和高级糖化终产物受体(RAGE))的过度激活。因此,转录因子核因子-kappa B 的活化程度降低,从而减少了 Vinpo 处理的高纤维脂肪泻/糖尿病大鼠体内促炎细胞因子 TNF-α 和 IL-6 的产生。与单独使用 Vinpo 治疗相比,乳酸杆菌益生菌作为 Vinpo 的辅助疗法,可通过加强共生微生物群的恢复,显著改善与疾病相关的炎症和氧化应激损伤,以及胰岛素抵抗和血脂异常。总之,将Vinpo和乳酸杆菌益生菌结合使用可能是限制人类非酒精性脂肪肝的一种成功方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Vinpocetine and Lactobacillus improve fatty liver in rats: role of adiponectin and gut microbiome.

Vinpocetine and Lactobacillus improve fatty liver in rats: role of adiponectin and gut microbiome.

Therapeutics that interfere with the damage/pathogen-associated molecular patterns (DAMPs/PAMPs) have evolved as promising candidates for hepatic inflammation like that occurring in non-alcoholic fatty liver disease (NAFLD). In the current study, we examined the therapeutic impact of the phosphodiesterase-1 inhibitor vinpocetine (Vinpo), alone or when combined with Lactobacillus, on hepatic abnormalities caused by a 13-week high-fat diet (HFD) and diabetes in rats. The results show that Vinpo (10 and 20 mg/kg/day) dose-dependently curbed HFD-induced elevation of liver injury parameters in serum (ALT, AST) and tissue histopathology. These effects were concordant with Vinpo's potential to ameliorate HFD-induced fibrosis (Histological fibrosis score, hydroxyproline, TGF-β1) and oxidative stress (MDA, NOx) alongside restoring the antioxidant-related parameters (GSH, SOD, Nrf-2, HO-1) in the liver. Mechanistically, Vinpo attenuated the hepatocellular release of DAMPs like high mobility group box (HMGB)1 alongside lowering the overactivation of the pattern recognition receptors including, toll-like receptor (TLR)4 and receptor for advanced glycation end-products (RAGE). Consequently, there was less activation of the transcription factor nuclear factor-kappa B that lowered production of the proinflammatory cytokines TNF-α and IL-6 in Vinpo-treated HFD/diabetes rats. Compared to Vinpo treatment alone, Lactobacillus probiotics as adjunctive therapy with Vinpo significantly improved the disease-associated inflammation and oxidative stress injury, as well as the insulin resistance and lipid profile abnormalities via enhancing the restoration of the symbiotic microbiota. In conclusion, combining Vinpo and Lactobacillus probiotics may be a successful approach for limiting NAFLD in humans.

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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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