基于山苍子精油的纳米乳液可增强多柔比星的细胞毒性和凋亡效应：一项体外研究。

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2024-11-01 Epub Date: 2024-08-02 DOI:10.1080/02652048.2024.2386287

Sahar M AlMotwaa, Waad A Al-Otaibi

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引用次数: 0

摘要

目的：本研究旨在探讨多柔比星（DOX）与山茱萸精油纳米制剂（SCEO-NANO）的新型组合对肝癌（HepG2）和结肠癌（HT29）细胞株的细胞毒作用：方法：通过高压均质法制备纳米乳液，然后用zetasizer和傅立叶变换红外光谱进行分析。在体外试验中使用 HepG2 和 HT29 细胞进行细胞凋亡检测：结果：与SCEO-NANO（尺寸：8.91 ± 0.02 nm；PDI = 0.1 ± 0.02）相比，DOX@SCEO-NANO/DOX的配方液滴尺寸增加到11.54 ± 0.02，分布均匀（PDI = 0.13 ± 0.01）。在这两种细胞中，DOX@SCEO-NANO/DOX 可显著减少菌落的形成。与 DOX 相比，apoprotein 蛋白在 HepG2 细胞中过度表达，Caspase-3 增加了 8.66 倍，Bax/Bcl-2 比率增加了 4.24 倍。在 HT29 细胞中，出现了 ROS 依赖性坏死和凋亡。DOX@SCEO-NANO/DOX与DOX相比，Caspase-3和Bax/Bcl-2比率的水平更高：结论：DOX@SCEO-NANO/DOX制剂具有靶向消灭结肠腺癌和肝癌细胞的潜力。

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Nano-emulsion based on Santolina chamaecyparissus essential oil potentiates the cytotoxic and apoptotic effects of Doxorubicin: an in vitro study.

Aim: This study was aimed at investigating the cytotoxic effect of a novel combination of doxorubicin (DOX) and nano-formulation of Santolina chamaecyparissus L. essential oil (SCEO-NANO) on hepatic (HepG2) and colon (HT29) cancer cell lines.

Methods: A nano-emulsion was prepared by high-pressure homogenisation, then analysed by zetasizer and Fourier transform infrared spectroscopy. HepG2 and HT29 cells were used in in vitro tests for apoptosis detection.

Results: Formulated droplet size increased in DOX@SCEO-NANO/DOX to 11.54 ± 0.02 with uniform distribution (PDI = 0.13 ± 0.01), when compared with SCEO-NANO (size: 8.91 ± 0.02 nm; PDI = 0.1 ± 0.02). In both cells, DOX@SCEO-NANO/DOX led to a considerable reduction in colony formation. Compared to DOX, apoprotein proteins were overexpressed in HepG2 cells, showing increases of 8.66-fold for caspase-3 and 4.24-fold for the Bax/Bcl-2 ratio. In HT29 cells, ROS-dependent necrosis and apoptosis were seen. Comparing DOX@SCEO-NANO/DOX versus DOX, greater levels of caspase-3 and the Bax/Bcl-2 ratio were observed.

Conclusion: The DOX@SCEO-NANO/DOX formulation showed potential for targeted eradication of colon adenocarcinoma and hepatocellular carcinoma cells.

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.