缺乏 sgg1 的小鼠巨细胞病毒对唾液腺的输入减少。

IF 3.6 4区医学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of General Virology Pub Date : 2024-08-01 DOI:10.1099/jgv.0.002013

Jiawei Ma, Kimberley Bruce, Philip G Stevenson, Helen E Farrell

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引用次数: 0

摘要

巨细胞病毒（CMV）通过唾液腺慢性脱落传播。目前还不清楚这与它们在体外表现出的广泛细胞滋养性之间的关系。人类 CMV（HCMV）只有在唾液腺感染后才会出现。因此，鼠 CMV（MCMV）可用于分析早期感染事件。它通过受感染的髓样细胞到达唾液腺。三个相邻的剪接基因被命名为 m131/129（MCK-2）、sgg1 和 sgg1.1，它们是 HCMV UL128/130/131 滋养决定因子的位置同源物。我们发现，sgg1 空缺突变体在受感染的髓细胞进入唾液腺方面存在缺陷，这种表型不同于缺乏 MCK-2 的 MCMV。这些数据表明唾液腺的定植过程是复杂的、多步骤的。

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Mouse cytomegalovirus lacking sgg1 shows reduced import into the salivary glands.

Cytomegaloviruses (CMVs) transmit via chronic shedding from the salivary glands. How this relates to the broad cell tropism they exhibit in vitro is unclear. Human CMV (HCMV) infection presents only after salivary gland infection is established. Murine CMV (MCMV) is therefore useful to analyse early infection events. It reaches the salivary glands via infected myeloid cells. Three adjacent spliced genes designated as m131/129 (MCK-2), sgg1 and sgg1.1, positional homologues of the HCMV UL128/130/131 tropism determinants, are implicated. We show that a sgg1 null mutant is defective in infected myeloid cell entry into the salivary glands, a phenotype distinct from MCMV lacking MCK-2. These data point to a complex, multi-step process of salivary gland colonization.

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来源期刊

Journal of General Virology 医学-病毒学

CiteScore

7.70

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： JOURNAL OF GENERAL VIROLOGY (JGV), a journal of the Society for General Microbiology (SGM), publishes high-calibre research papers with high production standards, giving the journal a worldwide reputation for excellence and attracting an eminent audience.