rmpD过表达可恢复微需氧菌介导的肺炎克雷伯氏菌粘液性抑制。

IF 3.2 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Wangnan Sun, Chengbo Rong, Liang Chen, Jiarui Li, Zhijing An, Jinglin Yue, Hengkun Wei, Kai Han, Mingxi Hua, Hui Zeng, Chen Chen
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引用次数: 0

摘要

目的:高病毒性肺炎克雷伯氏菌(hvKp)会引起健康人的侵入性社区获得性感染,而高黏液性(HMV)是与 hvKp 相关的主要表型。本研究探讨了微需氧环境对肺炎克雷伯菌粘度的影响:通过在微需氧和需氧环境下培养 25 株临床菌株,我们观察到在微需氧环境下粘液粘度明显降低。RNA测序和qRT-PCR显示,在微需氧条件下,胶囊合成基因(galf、orf2、wzi、wza、wzb、wzc、wcaj、manC、manB、ugd)和调控基因(rmpA、rmpD、rmpC)的表达下调。透射电子显微镜和印度墨水染色分析表明,与需氧条件相比,微氧条件下肺炎双球菌的囊膜厚度减少了一半。缺失 rmpD 和 rmpC 会导致有氧和微氧条件下 HMV 表型的丧失。然而,与微需氧条件下的野生型菌株相比,在微需氧条件下,只有rmpD过表达菌株(而非rmpC过表达菌株)的囊膜厚度显著增加:结论:微需氧条件可抑制肺炎克雷伯菌的粘液粘度,但这种抑制可通过改变rmpD的表达来克服,这表明rmpD在肺炎克雷伯菌的氧环境适应中具有特殊功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microaerobic-mediated suppression of Klebsiella pneumoniae mucoviscosity is restored by rmpD overexpression.

Aims: Hypervirulent Klebsiella pneumoniae (hvKp) causes invasive community-acquired infections in healthy individuals, and hypermucoviscosity (HMV) is the main phenotype associated with hvKp. This study investigates the impact of microaerobic environment availability on the mucoviscosity of K. pneumoniae.

Methods and results: By culturing 25 clinical strains under microaerobic and aerobic environments, we observed a notable reduction in mucoviscosity in microaerobic environments. RNA sequencing and qRT-PCR revealed downregulated expressions of capsule synthesis genes (galf, orf2, wzi, wza, wzb, wzc, wcaj, manC, manB, and ugd) and regulatory genes (rmpA, rmpD, and rmpC) under microaerobic conditions. Transmission electron microscopy and Indian ink staining analysis were performed, revealing that the capsular thickness of K. pneumoniae decreased by half in microaerobic conditions compared to aerobic conditions. Deletion of rmpD and rmpC caused the loss of the HMV phenotype in both aerobic and microaerobic conditions. However, compared to wild-type strain in microaerobic condition, only rmpD overexpression strain, and not rmpC overexpression strain, displayed a significant increase in capsule thickness in microaerobic conditions.

Conclusions: Microaerobic conditions can suppress the mucoviscosity of K. pneumoniae, but this suppression can be overcome by altering the expression of rmpD, indicating a specific function for rmpD in the oxygen environmental adaptation of K. pneumoniae.

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来源期刊
Journal of Applied Microbiology
Journal of Applied Microbiology 生物-生物工程与应用微生物
CiteScore
7.30
自引率
2.50%
发文量
427
审稿时长
2.7 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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