免疫检查点蛋白 PD-L1 和 TIM3 在小鼠和人类头颈部鳞状细胞癌中的表达。

IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Areeg Elmusrati, Cun-Yu Wang
{"title":"免疫检查点蛋白 PD-L1 和 TIM3 在小鼠和人类头颈部鳞状细胞癌中的表达。","authors":"Areeg Elmusrati,&nbsp;Cun-Yu Wang","doi":"10.1111/eos.13010","DOIUrl":null,"url":null,"abstract":"<p>The aim of this study was to examine the expression of programmed death-ligand 1 (PD-L1) and of T cell immunoglobulin and mucin domain-containing protein (TIM3) in oral epithelial dysplasia and head and neck squamous cell carcinoma (HNSCC). Mouse HNSCC was induced with 4-nitroquinoline-1 oxide (4NQO). Oral epithelial dysplastic lesions, carcinoma in situ and HNSCC lesions were stained with anti-PD-L1 and TIM3 antibodies. The expression of PD-L1 and TIM3 in tumor cells and immune cells was semiquantitatively measured and compared. In parallel, human dysplasia and HNSCC were stained with anti-PD-L1 and anti-TIM3. The expression pattern of PD-L1<sup>+</sup> and TIM3<sup>+</sup> cells was further compared. In human and mouse samples both PD-L1 and TIM3 were found to be expressed in neoplastic and immune cells in HNSCC, but not in dysplasia. There was no significant difference in PD-L1 and TIM3 expression between metastatic and nonmetastatic HNSCC. We conclude that the 4NQO-induced mouse HNSCC model may be an excellent preclinical model for immune checkpoint therapy.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"132 5","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The expression of immune checkpoint proteins PD-L1 and TIM3 in mouse and human head and neck squamous cell carcinoma\",\"authors\":\"Areeg Elmusrati,&nbsp;Cun-Yu Wang\",\"doi\":\"10.1111/eos.13010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The aim of this study was to examine the expression of programmed death-ligand 1 (PD-L1) and of T cell immunoglobulin and mucin domain-containing protein (TIM3) in oral epithelial dysplasia and head and neck squamous cell carcinoma (HNSCC). Mouse HNSCC was induced with 4-nitroquinoline-1 oxide (4NQO). Oral epithelial dysplastic lesions, carcinoma in situ and HNSCC lesions were stained with anti-PD-L1 and TIM3 antibodies. The expression of PD-L1 and TIM3 in tumor cells and immune cells was semiquantitatively measured and compared. In parallel, human dysplasia and HNSCC were stained with anti-PD-L1 and anti-TIM3. The expression pattern of PD-L1<sup>+</sup> and TIM3<sup>+</sup> cells was further compared. In human and mouse samples both PD-L1 and TIM3 were found to be expressed in neoplastic and immune cells in HNSCC, but not in dysplasia. There was no significant difference in PD-L1 and TIM3 expression between metastatic and nonmetastatic HNSCC. We conclude that the 4NQO-induced mouse HNSCC model may be an excellent preclinical model for immune checkpoint therapy.</p>\",\"PeriodicalId\":11983,\"journal\":{\"name\":\"European Journal of Oral Sciences\",\"volume\":\"132 5\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Journal of Oral Sciences\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/eos.13010\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Oral Sciences","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/eos.13010","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0

摘要

本研究旨在检测程序性死亡配体1(PD-L1)和含T细胞免疫球蛋白和粘蛋白结构域蛋白(TIM3)在口腔上皮发育不良和头颈部鳞状细胞癌(HNSCC)中的表达。用 4-硝基喹啉-1 氧化物(4NQO)诱导小鼠 HNSCC。用抗 PD-L1 和 TIM3 抗体对口腔上皮发育不良病变、原位癌和 HNSCC 病变进行染色。对肿瘤细胞和免疫细胞中 PD-L1 和 TIM3 的表达进行半定量测定和比较。同时,用抗 PD-L1 和抗 TIM3 对人类发育不良和 HNSCC 进行染色。进一步比较了 PD-L1+ 和 TIM3+ 细胞的表达模式。在人类和小鼠样本中发现,PD-L1 和 TIM3 在 HNSCC 的肿瘤细胞和免疫细胞中均有表达,但在发育不良中没有表达。在转移性和非转移性 HNSCC 中,PD-L1 和 TIM3 的表达没有明显差异。我们的结论是,4NQO诱导的小鼠HNSCC模型可能是免疫检查点疗法的绝佳临床前模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The expression of immune checkpoint proteins PD-L1 and TIM3 in mouse and human head and neck squamous cell carcinoma

The aim of this study was to examine the expression of programmed death-ligand 1 (PD-L1) and of T cell immunoglobulin and mucin domain-containing protein (TIM3) in oral epithelial dysplasia and head and neck squamous cell carcinoma (HNSCC). Mouse HNSCC was induced with 4-nitroquinoline-1 oxide (4NQO). Oral epithelial dysplastic lesions, carcinoma in situ and HNSCC lesions were stained with anti-PD-L1 and TIM3 antibodies. The expression of PD-L1 and TIM3 in tumor cells and immune cells was semiquantitatively measured and compared. In parallel, human dysplasia and HNSCC were stained with anti-PD-L1 and anti-TIM3. The expression pattern of PD-L1+ and TIM3+ cells was further compared. In human and mouse samples both PD-L1 and TIM3 were found to be expressed in neoplastic and immune cells in HNSCC, but not in dysplasia. There was no significant difference in PD-L1 and TIM3 expression between metastatic and nonmetastatic HNSCC. We conclude that the 4NQO-induced mouse HNSCC model may be an excellent preclinical model for immune checkpoint therapy.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
European Journal of Oral Sciences
European Journal of Oral Sciences 医学-牙科与口腔外科
CiteScore
3.50
自引率
5.30%
发文量
61
审稿时长
2 months
期刊介绍: The European Journal of Oral Sciences is an international journal which publishes original research papers within clinical dentistry, on all basic science aspects of structure, chemistry, developmental biology, physiology and pathology of relevant tissues, as well as on microbiology, biomaterials and the behavioural sciences as they relate to dentistry. In general, analytical studies are preferred to descriptive ones. Reviews, Short Communications and Letters to the Editor will also be considered for publication. The journal is published bimonthly.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信