基因组规模分析揭示克隆猴的障碍

IF 1.2 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cellular reprogramming Pub Date : 2024-08-01 DOI:10.1089/cell.2024.0048

Marcelo Tigre Moura

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引用次数: 0

摘要

通过体细胞核移植（SCNT）进行克隆对恒河猴来说仍然具有挑战性，主要是因为其效率低和新生儿死亡。基因组规模的分析表明，恒河猴 SCNT 胚胎显示出广泛的 DNA 甲基化和转录改变，从而包括与胎盘功能障碍相关的基因组印记缺失。将克隆囊胚中的内细胞团（ICM）移植到缺失 ICM 的受精胚胎中，可挽救胎盘功能不全的问题，克隆出的恒河猴成年后无明显异常。

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Genome-Scale Analyses Reveal Roadblocks to Monkey Cloning.

Cloning by somatic cell nuclear transfer (SCNT) remained challenging for Rhesus monkeys, mostly due to its low efficiency and neonatal death. Genome-scale analyses revealed that monkey SCNT embryos displayed widespread DNA methylation and transcriptional alterations, thus including loss of genomic imprinting that correlated with placental dysfunction. The transfer of inner cell masses (ICM) from cloned blastocysts into ICM-depleted fertilized embryos rescued placental insufficiency and gave rise to a cloned Rhesus monkey that reached adulthood without noticeable abnormalities.

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来源期刊

Cellular reprogramming CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

2.50

自引率

6.20%

发文量

审稿时长

3 months

期刊介绍： Cellular Reprogramming is the premier journal dedicated to providing new insights on the etiology, development, and potential treatment of various diseases through reprogramming cellular mechanisms. The Journal delivers information on cutting-edge techniques and the latest high-quality research and discoveries that are transforming biomedical research. Cellular Reprogramming coverage includes: Somatic cell nuclear transfer and reprogramming in early embryos Embryonic stem cells Nuclear transfer stem cells (stem cells derived from nuclear transfer embryos) Generation of induced pluripotent stem (iPS) cells and/or potential for cell-based therapies Epigenetics Adult stem cells and pluripotency.