Wen Hao Tian, Wen Yan Cai, Chun Quan Zhu, Ya Li Kong, Xiao Chuang Cao, Lian Feng Zhu, Jia Yuan Ye, Jun Hua Zhang, Shao Jian Zheng
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引用次数: 0
摘要
钙(Ca)是植物生长和适应胁迫所必需的元素,但在酸性土壤中,钙的供应往往受到限制,对作物生产构成重大威胁。植物对钙缺乏的适应机制错综复杂,但人们对其的了解仍然很有限。在这里,我们发现拟南芥中钙缺乏增强的转录因子 SENSITIVE TO PROTON RHIZOTOXICITY 1(STOP1)的核积累赋予了拟南芥对钙缺乏的耐受性,在 stop1 突变体中,钙缺乏引发的全局转录反应在很大程度上受损。值得注意的是,STOP1 通过直接结合 CATION/Ca2+ EXCHANGER 1(CCX1)的启动子区域,激活了钙剥夺诱导的 CATION/Ca2+ EXCHANGER 1(CCX1)的表达,从而促进 Ca2+ 从内质网流出到细胞质,维持 Ca 的平衡。因此,在stop1突变体中组成型表达CCX1可通过增加芽中的Ca含量来部分挽救Ca缺乏表型。这些发现揭示了 STOP1-CCX1 轴在植物适应低 Ca 过程中的关键作用,为改善酸性土壤中植物的 Ca 营养提供了可供选择的操作策略,并扩展了我们对 STOP1 多方面作用的认识。
STOP1 regulates CCX1-mediated Ca2+ homeostasis for plant adaptation to Ca2+ deprivation
Calcium (Ca) is essential for plant growth and stress adaptation, yet its availability is often limited in acidic soils, posing a major threat to crop production. Understanding the intricate mechanisms orchestrating plant adaptation to Ca deficiency remains elusive. Here, we show that the Ca deficiency-enhanced nuclear accumulation of the transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1) in Arabidopsis thaliana confers tolerance to Ca deprivation, with the global transcriptional responses triggered by Ca deprivation largely impaired in the stop1 mutant. Notably, STOP1 activates the Ca deprivation-induced expression of CATION/Ca2+EXCHANGER 1 (CCX1) by directly binding to its promoter region, which facilitates Ca2+ efflux from endoplasmic reticulum to cytosol to maintain Ca homeostasis. Consequently, the constitutive expression of CCX1 in the stop1 mutant partially rescues the Ca deficiency phenotype by increasing Ca content in the shoots. These findings uncover the pivotal role of the STOP1-CCX1 axis in plant adaptation to low Ca, offering alternative manipulating strategies to improve plant Ca nutrition in acidic soils and extending our understanding of the multifaceted role of STOP1.
期刊介绍:
Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.