Novel production strategy of drug-encapsulated biodegradable scaffolds for remediation of hidradenitis suppurativa

Abstract

This study examined producing three-dimensional printed CS scaffolds coated with rifampicin (RIF)-loaded poly(lactic acid) (PLA) microparticles to treat hidradenitis suppurativa lesions. The morphology of the particle-coated scaffolds was examined; it was observed that the particles obtained were monodisperse and of an ideal size for the intended application. According to the drug-release results, the release of drugs was completed in 24 h from the CS/PLA-2RIF scaffold and in 48 h from the CS/PLA-3RIF scaffold. Antimicrobial analysis was performed using Escherichia coli and Staphylococcus aureus; the scaffolds showed effectiveness against these bacterial strains. Per MTT analysis, the scaffolds had no toxic effects; it was noted that the cells easily adapted to the scaffold. The results from the encapsulated rifampicin-loaded PLA-coated CS scaffolds indicate that these scaffolds are a promising alternative method for treating hidradenitis suppurativa.

Impact statement

This study evaluated the creation of three-dimensional (3D) printed CS scaffolds that were coated with rifampicin (RIF)-loaded poly(lactic acid) (PLA) microparticles; the goal of this approach involves the treatment of the lesions associated with hidradenitis suppurativa (HS). The drug-release results indicate that the release of the drugs was completed in 24 h from the CS/PLA-2RIF scaffold and in 48 h from the CS/PLA-3RIF scaffold. Antimicrobial analysis was conducted using Escherichia coli and Staphylococcus aureus; the scaffolds showed effectiveness against these bacterial strains. Larger zones were demonstrated in testing the scaffolds with gram-positive bacteria; smaller zones were demonstrated in testing the scaffolds with gram-negative bacteria. The PLS/CS scaffold was associated with a small inhibition zone; this finding was attributed to the natural antimicrobial effect of CS. The MTT results suggest that scaffolds were not associated with toxic effects. The results of this study suggest that the wound dressing obtained by combining 3D printing and EHDA methods could provide an alternative approach to treat HS.

Graphical abstract