利用质谱成像技术（MSI）鉴定植物化合物

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-07-30 DOI:10.3390/metabo14080419

Nancy Shyrley García-Rojas, Carlos Daniel Sierra-Álvarez, Hilda E. Ramos-Aboites, Abigail Moreno-Pedraza, Robert Winkler

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引用次数: 0

摘要

植物代谢物的存在和定位表明了生理过程，例如在生物和非生物胁迫条件下的生理过程。此外，植物部分的化学成分与其作为食品或药物应用的质量有关。质谱成像（MSI）已成为一种流行的分析技术，用于探索和观察植物分子在组织内的空间分布。本综述概述了用于绘制和鉴定植物组织中代谢物的质谱方法。我们介绍了真空和常温电离方法的优点和缺点，并考虑了直接和间接方法。最后，我们讨论了目前在注释和鉴定分子方面的局限性以及未来研究的前景。

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Identification of Plant Compounds with Mass Spectrometry Imaging (MSI)

The presence and localization of plant metabolites are indicative of physiological processes, e.g., under biotic and abiotic stress conditions. Further, the chemical composition of plant parts is related to their quality as food or for medicinal applications. Mass spectrometry imaging (MSI) has become a popular analytical technique for exploring and visualizing the spatial distribution of plant molecules within a tissue. This review provides a summary of mass spectrometry methods used for mapping and identifying metabolites in plant tissues. We present the benefits and the disadvantages of both vacuum and ambient ionization methods, considering direct and indirect approaches. Finally, we discuss the current limitations in annotating and identifying molecules and perspectives for future investigations.

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.