通过核磁共振波谱和液相色谱-高分辨质谱法研究骨肉瘤中的脂质组学：一项试点研究

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-07-28 DOI:10.3390/metabo14080416

João Guilherme de Moraes Pontes, Milka Jadranin, Márcia Regina Assalin, Melissa Quintero Escobar, Danijela Stanisic, Tássia Brena Barroso Carneiro Costa, André van Helvoort Lengert, Érica Boldrini, Sandra Regina Morini da Silva, Daniel Onofre Vidal, Leticia Huan Bacellar Liu, Mariana Maschietto, Ljubica Tasic

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引用次数: 0

摘要

癌症是一种复杂的疾病，也会影响年轻人群；然而，它却造成了儿童和青少年相对较高的死亡率，尤其是在中低收入国家（LMICs）。此外，针对这一年龄段人群的脂质体研究也很少。因此，我们采用 NMR 和 LC-MS 技术分析了骨肉瘤（osteosarcoma）年轻患者（12 至 35 岁）的血清样本，并将其脂质组学与健康对照组（HC 组）样本的脂质组学进行了比较。此外，有转移和无转移的骨肉瘤患者之间的脂质组学差异表明，骨肉瘤患者的甘油磷酸胆碱（GPC）和甘油磷脂（GPL）水平较高，而在转移过程中胆固醇、胆碱、多不饱和脂肪酸（PUFA）和甘油则有所增加。在外周血中检测到的这些差异可用作液体活检的生物标记物。

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Lipidomics by Nuclear Magnetic Resonance Spectroscopy and Liquid Chromatography–High-Resolution Mass Spectrometry in Osteosarcoma: A Pilot Study

Cancer is a complex disease that can also affect the younger population; however, it is responsible for a relatively high mortality rate of children and youth, especially in low- and middle-income countries (LMICs). Besides that, lipidomic studies in this age range are scarce. Therefore, we analyzed blood serum samples from young patients (12 to 35 years) with bone sarcoma (osteosarcoma) and compared their lipidomics to the ones from the control group of samples, named healthy control (HC group), using NMR and LC-MS techniques. Furthermore, differences in the lipidomic profiles between OS patients with and without metastasis indicate higher glycerophosphocholine (GPC) and glycerophospholipid (GPL) levels in osteosarcoma and increased cholesterol, choline, polyunsaturated fatty acids (PUFAs), and glycerols during the metastasis. These differences, detected in the peripheral blood, could be used as biomarkers for liquid biopsy.

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.