利用 Kappaphycus alvarezii 硫酸多糖提取物和植物生长调节剂体外再生具有可控酚类分泌的棉花(Gossypium hirsutum L.)栽培品种 KC3

IF 2.2 3区 生物学 Q4 CELL BIOLOGY
Packiaraj Gurusaravanan, Sathasivam Vinoth, Rajkumar Vasanthkumar, Muthukrishnan Arun, Muthukrishnan Saradhadevi, Subiramani Sivakumar, Narayanasamy Jayabalan
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引用次数: 0

摘要

通过使用植物生长调节剂(PGRs)结合海藻多糖(SP)提取物,为精英棉花栽培品种 KC3 开发了一种高效且可重复的体外再生方案。傅立叶变换红外光谱(FT-IR)和碳-13(13C)核磁共振(NMR)光谱分析证实了海藻提取物中多糖的存在。对提取的 SP 提取物的体外植物再生功效进行了测试。在添加了 4.0% 葡萄糖、1.5 mg L-1 噻虫隆(TDZ)、0.6 mg L-1 2,4-二氯苯氧乙酸(2,4-D)和 30.0 mg L-1 SP 的 Murashige 和 Skoog(MS)培养基中,下胚轴外植体获得的胼胝体频率最高(89.4%)。值得注意的是,PGR 和 SP 强化培养基抑制了外植体的酚类排泄。发育良好的黄绿色易碎胼胝体被转移到芽萌发培养基上。添加了 4.0% 葡萄糖、2.0 mg L-1 6-(γ,γ-二甲基烯丙基氨基)嘌呤(2iP)、1.0 mg L-1 可可碱(KIN)、1.0 mg L-1 6-苄基氨基嘌呤(BA)和 40.0 mg L-1 SP 的 MS 培养基显示出最大的反应(85.2%),每个胼胝体产生 9.5 个芽。伸长的芽在根诱导培养基上培养,该培养基由含有吲哚-3-乙酸(IAA)、吲哚-3-丁酸(IBA)、1-萘乙酸(NAA)和 SP 的 Murashige 和 Skoog(MS)盐组成。结果表明,在添加了 0.6 毫克/升 IBA 和 30.0 毫克/升 SP 的 MS 培养基上,根的数量最多(12.9 条/芽),长度为 8.6 厘米。因此,添加天然生物刺激剂的改良 MS 培养基通过中和外植体分泌的酚类化合物的影响,更有潜力和更可靠地用于植物的体外再生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

In vitro regeneration of cotton (Gossypium hirsutum L.) cultivar KC3 with controlled phenolic secretion by using Kappaphycus alvarezii sulfated polysaccharide extract and plant growth regulators

In vitro regeneration of cotton (Gossypium hirsutum L.) cultivar KC3 with controlled phenolic secretion by using Kappaphycus alvarezii sulfated polysaccharide extract and plant growth regulators

An efficient and reproducible in vitro regeneration protocol was developed for elite cotton cultivar KC3 by using plant growth regulators (PGRs) in combination with seaweed polysaccharide (SP) extracts. The existence of polysaccharide in seaweed extract was confirmed by Fourier transform infrared spectroscopy (FT-IR) and carbon-13 (13C) nuclear magnetic resonance (NMR) spectroscopy analysis. The extracted SP extract efficacy was tested for in vitro plant regeneration. The maximum callus frequency (89.4%) was obtained from hypocotyl explant in the Murashige and Skoog (MS) medium supplemented with 4.0% glucose, 1.5 mg L−1 thidiazuron (TDZ), 0.6 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D), and 30.0 mg L−1 SP. Remarkably, PGR- and SP-fortified medium inhibits the phenolic excretion from the explants. The well-developed yellow green friable texture of callus was transferred to shoot initiation medium. MS medium fortified with 4.0% glucose, 2.0 mg L−1 6-(γ,γ-dimethylallylamino) purine (2iP), 1.0 mg L−1 kinetin (KIN), 1.0 mg L−1 6-benzylaminopurine (BA), and 40.0 mg L−1 SP has shown maximum response (85.2%), and it produced 9.5 shoots per callus. The elongated shoots were cultured on root induction medium which consists of Murashige and Skoog (MS) salts with indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA), and SP. The results revealed that the maximum number of roots (12.9 per shoot) with 8.6 cm in length was achieved on MS medium supplemented with 0.6 mg L−1 IBA, combined with 30.0 mg L−1 SP. Therefore, modified MS medium with natural bio-stimulant has more potential and is more reliable for in vitro regeneration of plants by neutralizing the effects of phenolic compounds secreted by the explants.

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来源期刊
CiteScore
5.00
自引率
7.70%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.
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