{"title":"三种嗜油菌株对原油中烷烃的生物降解差异","authors":"Carlos Costa, Nicolás Millán","doi":"10.1016/j.ibiod.2024.105864","DOIUrl":null,"url":null,"abstract":"<div><p><em>Ex situ</em> bioremediation of crude oil in bioreactors by oleophilic bacteria permits to regulate temperature, nutrients and oxygen and the results in petroleum removal are much more effective than <em>in situ</em> bioremediation. This article quantify the biodegradation of alkanes which conform crude oil in bioreactors by three oleophilic strains. Samples were collected from the bioreactors and extracted in dichloromethane (10:1 aqueous/organic v/v) for amplification in GC-FID analysis of individual alkanes. 94% of crude oil (initial concentration 8.6 g l<sup>−1</sup>) is removed in 24 days by <em>Bacillus licheniformis</em> and in 38 days by <em>Pseudomonas putida</em> and <em>Paenibacillus glucanolyticus</em>. Higher biodegradation rates (9.0–26.5 mg l<sup>−1</sup>d<sup>−1</sup>) are reached for the lightest fraction of crude oil by <em>Bacillus licheniformis</em> (C10–C16), and much lower for C22–C25 alkanes (4.4–8.9 mg l<sup>−1</sup>d<sup>−1</sup>). <em>Pseudomonas putida</em> and <em>Paenibacillus glucanolyticus</em> degrade a wide range of alkanes at 1.6–9.6 mg l<sup>−1</sup>d<sup>−1</sup>, with preference over C8–C16 hydrocarbons. Heptane and octane (alkanes cited to be toxic) are difficult for biodegradation in two strains (<em>B. licheniformis</em> and <em>P. putida</em>) and a recalcitrant “window” C18–C21 is observed for the three oleophilic strains in the alkane range studied.</p></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"194 ","pages":"Article 105864"},"PeriodicalIF":4.1000,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Differential biodegradation of alkanes in crude oil by three oleophilic strains\",\"authors\":\"Carlos Costa, Nicolás Millán\",\"doi\":\"10.1016/j.ibiod.2024.105864\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Ex situ</em> bioremediation of crude oil in bioreactors by oleophilic bacteria permits to regulate temperature, nutrients and oxygen and the results in petroleum removal are much more effective than <em>in situ</em> bioremediation. This article quantify the biodegradation of alkanes which conform crude oil in bioreactors by three oleophilic strains. Samples were collected from the bioreactors and extracted in dichloromethane (10:1 aqueous/organic v/v) for amplification in GC-FID analysis of individual alkanes. 94% of crude oil (initial concentration 8.6 g l<sup>−1</sup>) is removed in 24 days by <em>Bacillus licheniformis</em> and in 38 days by <em>Pseudomonas putida</em> and <em>Paenibacillus glucanolyticus</em>. Higher biodegradation rates (9.0–26.5 mg l<sup>−1</sup>d<sup>−1</sup>) are reached for the lightest fraction of crude oil by <em>Bacillus licheniformis</em> (C10–C16), and much lower for C22–C25 alkanes (4.4–8.9 mg l<sup>−1</sup>d<sup>−1</sup>). <em>Pseudomonas putida</em> and <em>Paenibacillus glucanolyticus</em> degrade a wide range of alkanes at 1.6–9.6 mg l<sup>−1</sup>d<sup>−1</sup>, with preference over C8–C16 hydrocarbons. Heptane and octane (alkanes cited to be toxic) are difficult for biodegradation in two strains (<em>B. licheniformis</em> and <em>P. putida</em>) and a recalcitrant “window” C18–C21 is observed for the three oleophilic strains in the alkane range studied.</p></div>\",\"PeriodicalId\":13643,\"journal\":{\"name\":\"International Biodeterioration & Biodegradation\",\"volume\":\"194 \",\"pages\":\"Article 105864\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-07-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Biodeterioration & Biodegradation\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0964830524001355\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Biodeterioration & Biodegradation","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0964830524001355","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Differential biodegradation of alkanes in crude oil by three oleophilic strains
Ex situ bioremediation of crude oil in bioreactors by oleophilic bacteria permits to regulate temperature, nutrients and oxygen and the results in petroleum removal are much more effective than in situ bioremediation. This article quantify the biodegradation of alkanes which conform crude oil in bioreactors by three oleophilic strains. Samples were collected from the bioreactors and extracted in dichloromethane (10:1 aqueous/organic v/v) for amplification in GC-FID analysis of individual alkanes. 94% of crude oil (initial concentration 8.6 g l−1) is removed in 24 days by Bacillus licheniformis and in 38 days by Pseudomonas putida and Paenibacillus glucanolyticus. Higher biodegradation rates (9.0–26.5 mg l−1d−1) are reached for the lightest fraction of crude oil by Bacillus licheniformis (C10–C16), and much lower for C22–C25 alkanes (4.4–8.9 mg l−1d−1). Pseudomonas putida and Paenibacillus glucanolyticus degrade a wide range of alkanes at 1.6–9.6 mg l−1d−1, with preference over C8–C16 hydrocarbons. Heptane and octane (alkanes cited to be toxic) are difficult for biodegradation in two strains (B. licheniformis and P. putida) and a recalcitrant “window” C18–C21 is observed for the three oleophilic strains in the alkane range studied.
期刊介绍:
International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.