用于灵敏、连续检测痕量分子的膜过滤器集成微流控装置中的 SERS 活性核心卫星纳米结构

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
Li-An Wu, Kai-Ting Hsieh, Chien-Shen Lin, Yuh-Lin Wang, Yih-Fan Chen
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引用次数: 0

摘要

我们开发了一种表面增强拉曼散射(SERS)活性等离子体核心-卫星纳米结构,并将其纳入膜过滤集成微流控装置,用于连续监测溶液中的分子。核心-卫星纳米结构是通过将高密度的金纳米粒子(AuNPs)固定在二氧化硅珠上,在AuNPs之间形成许多纳米间隙而制成的。通过添加银(Ag)壳对纳米间隙的大小进行微调,以优化 SERS 活性。此外,纳米粒子的封端剂柠檬酸盐分子被碱卤化物置换。这种置换不仅降低了柠檬酸盐的 SERS 信号,还增强了对目标分子的吸附。经碱卤化物处理的核心-卫星纳米结构被积聚到集成到微流控装置中的膜过滤器上,作为均匀、灵敏的 SERS 基底。通过增加流经膜过滤器的样品溶液体积,我们增加了纳米结构上吸附的分子数量,从而放大了其特征拉曼峰的强度。我们的微流控 SERS 设备在孔雀石绿浓度低至 500 fM 时就能对其进行连续 SERS 检测。总之,虽然已有各种核心卫星纳米结构和微流控 SERS 装置的报道,但在我们的研究中,含膜过滤器的微流控装置与核心卫星纳米结构的整合促进了灵敏和连续的分子检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

SERS-active core-satellite nanostructures in a membrane filter-integrated microfluidic device for sensitive and continuous detection of trace molecules

SERS-active core-satellite nanostructures in a membrane filter-integrated microfluidic device for sensitive and continuous detection of trace molecules

We developed a surface-enhanced Raman scattering (SERS)-active plasmonic core-satellite nanostructure and incorporated it into a membrane filter-integrated microfluidic device for continuous monitoring of molecules in solution. The core-satellite nanostructures were fabricated by immobilizing a high number density of gold nanoparticles (AuNPs) on silica beads.to create many nanogaps among the AuNPs. The sizes of the nanogaps were fine-tuned by adding a silver (Ag) shell to optimize the SERS activity. In addition, citrate molecule, the capping agent of the nanoparticles, was displaced by alkali halides. The displacement not only reduced the SERS signals of citrate but also enhanced the adsorption of target molecules. The alkali halide-treated core-satellite nanostructures were accumulated onto a membrane filter integrated into a microfluidic device, serving as a uniform and sensitive SERS substrate. By increasing the volume of the sample solution flowing through the membrane filter, we increased the number of molecules adsorbed to the nanostructures, amplifying the intensities of their characteristic Raman peaks. Our microfluidic SERS device demonstrated continuous SERS detection of malachite green at a concentration as low as 500 fM. In summary, while various core-satellite nanostructures and microfluidic SERS devices have been reported, the integration of the membrane filter-containing microfluidic device with the core-satellite nanostructures facilitated sensitive and continuous molecule detection in our study.

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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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