Pro-Oxidant/ Antioxidant Balance Correlates with Red Blood Cell Indices and Anemia Severity in the Anemic Patients.

Background: The oxidant/ antioxidant balance is disrupted in anemia. Antioxidant capacity depends on antioxidant enzyme activity and some trace elements. This study aimed to evaluate oxidant/ antioxidant status and its correlation with red blood cell indices and anemia severity in anemic patients.

Methods: Blood samples were taken from 90 anemic patients and 95 healthy people. Circulatory miR-122 was assayed by real-time PCR. Malondialdehyde (MDA), pro-oxidant/ antioxidant balance (PAB), supper oxide demitasse (SOD), glutathione peroxidase (GPxs) activity, total antioxidant capacity (TAC), and zinc were measured by colorimetric method. Selenium was also determined using atomic absorption.

Results: Selenium and zinc decreased significantly in the case group (**P=0.004 and ***P=0.000). The amount of miR-122 up-regulated in the anemia (**P=0.003). MDA was significantly raised in the case vs control (***P=0.0002). PAB was higher in the case group (**P=0.005). SOD and GPxs activity was decreased along with TAC in anemic patients (*P=0.02, **P=0.008, *P=0.038). Zinc and PAB levels correlated with some red blood cell indices. PAB was associated with anemia severity.

Conclusions: Increased PAB and decreased zinc/selenium increased oxidant levels in anemic patients. RBC indices and anemia severity were correlated with oxidant/ antioxidant somewhere.