利用孢子型益生菌对抗表皮葡萄球菌的顽固细胞

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Saeid Kheirjou, Farzaneh Hosseini, Framarz Masjedian Jazi, Elham Siasi Torbati
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引用次数: 0

摘要

背景:本研究采用孢子形成的益生菌来消除表皮葡萄球菌生物膜,并检测了应激反应相关基因的表达:本研究利用孢子形成的益生菌根除表皮葡萄球菌生物膜,并检测了涉及应激反应的基因的存在和表达:聚合酶链反应(PCR)检测表皮葡萄球菌 ATCC 12228 的 rpoS、relA 和 mazF 基因。通过微孔板(MTP)检测法研究生物膜的生成。使用 100 倍最小抑菌浓度(MIC)的庆大霉素诱导浮游和生物膜细菌细胞中的固着细胞。在 2、8 和 24 小时的不同时间间隔内,使用实时 PCR 检测法评估 rpoS、relA 和 mazF 基因的表达。然后,用 1、0.5 和 0.25 µg/ml 的凝结芽孢杆菌培养上清液稀释液来消灭宿主细胞,并测定菌落数:结果:表皮葡萄球菌在接触 50 µg/ml 庆大霉素 7 小时后在浮游生物结构中形成宿主细胞,5 小时后在生物膜结构中形成宿主细胞。与对照组相比,生物膜结构中 mazF 和 rpoS 的表达量以及宿主细胞中 rpoS 和 relA 的表达量均显著升高(p< 0.05)。在暴露于 1 µg/ml 和 0.5 µg/ml 的凝结球菌上清液后,宿主细胞的数量分别减少了对数 2.4 和对数 0.8,但在浓度为 0.25 µg/ml 时则没有减少:结果表明,含有益生菌分泌代谢产物的益生菌上清液可作为一种新型方法来对付顽固细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Employment of Spore-Forming Probiotics to Combat Persister Cells of Staphylococcus Epidermidis.

Background: In this study, spore-forming probiotics were employed to eradicate Staphylococcus epidermidis biofilms and the presence and expression of genes involved in stress response was examined.

Methods: Polymerase chain reaction (PCR) assay was used to detect rpoS, relA and mazF genes in S. epidermidis ATCC 12228. Biofilm production was investigated by microtiter plate (MTP) assay. 100X minimum inhibitory concentration (MIC) of gentamycin was used to induce persister cells in planktonic and biofilm bacterial cells. The expression of rpoS, relA, and mazF genes was assessed at different time intervals of 2, 8, and 24 h using real-time PCR assay. Then, dilutions of 1, 0.5, and 0.25 µg/ml of the supernatant of Bacillus coagulans culture was used to eradicate the persister cells and the number of colonies was determined.

Results: Persister cells of S. epidermidis were formed after 7 h in planktonic and 5 h in the biofilm structure after exposure to 50 µg/ml of gentamycin. The expression of mazF and rpoS in biofilm structure and the expression of rpoS and relA in persister cells were significantly higher compared to the control (p< 0.05). The number of persister cells showed a reduction of log 2.4 and log 0.8 after exposure to 1 and 0.5 µg/ml B. coagulans supernatant, respectively, but no reduction was observed at the concentration of 0.25 µg/ml.

Conclusion: The results showed that the supernatant of probiotics containing their secretive metabolites can be used as a novel approach to combat persister cells.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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