纳米氧化锌颗粒通过调控凋亡基因改善大鼠肝脏缺血再灌注损伤模型的组织学改变

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Maryam Jafar Sameri, Feryal Savari, Seyyed Ali Mard, Anahita Rezaie, Mojtaba Kalantar
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引用次数: 0

摘要

背景:器官缺血再灌注(IR)是一种常见的临床症状,与创伤手术、器官移植和心肌缺血等各种情况有关。目前针对 IR 损伤的治疗方法存在局限性,而纳米技术,尤其是氧化锌纳米粒子(ZnO NPs),为疾病诊断和治疗提供了新方法。本研究探讨了氧化锌纳米粒子对大鼠肝脏缺血再灌注损伤的保护和抗凋亡作用:方法:将48只雄性大鼠分为6组:假组、ZnO5组、ZnO10组、缺血再灌注组(IR)、IR+ZnO5组和IR+ZnO10组。通过肝酶(AST、ALT、胆红素、ALP)、生化指标(TAC、TNF-α和MDA)、分子检测(Bcl2、BAX)和组织病理学评估(H&E、TUNEL)评估氧化锌氮氧化物的保护作用:结果:ZnO5 和 ZnO10 的预处理改善了红外肝损伤的肝功能,降低了氧化剂(P = 0.03)和炎症介质的水平,并减少了细胞凋亡(P = 0)。与 ZnO5 相比,ZnO10 对缺血再灌注损伤的影响更大。组织病理学检查还显示,IR+ZnO5 组和 IR+ZnO10 组的组织病理学改变呈剂量依赖性减少:结论:服用 ZnO5 和 ZnO10 可改善 IR 后的肝功能。本研究结果表明,氧化锌氮氧化物对大鼠肝脏缺血再灌注损伤中的氧化应激和细胞凋亡具有保护作用。这些结果可能对开发先进的缺血再灌注治疗方法具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Zinc Oxide Nanoparticles Ameliorate Histological Alterations Through Apoptotic Gene Regulation in Rat Model of Liver Ischemia-Reperfusion Injury.

Background: Organ ischemia-reperfusion (IR) is a common clinical condition associated with various situations such as trauma surgery, organ transplantation, and myocardial ischemia. Current therapeutic methods for IR injury have limitations, and nanotechnology, particularly zinc oxide nanoparticles (ZnO NPs), offers new approaches for disease diagnosis and treatment. In this study, we investigated the protective and anti-apoptotic effects of ZnO NPs in liver ischemia-reperfusion (IR) injury in rats.

Methods: Forty-eight male rats were divided into six groups: sham, ZnO5, ZnO10, ischemia-reperfusion (IR), IR+ZnO5, and IR+ZnO10. The protective effect of ZnO NPs was evaluated by liver enzymes (AST, ALT, Bilirubin, ALP), biochemical (TAC, TNF-α, and MDA), molecular examinations (Bcl2, BAX), and histopathological evaluations (H&E, TUNEL).

Results: Pre-treatment with ZnO5 and ZnO10 improved hepatic function in IR liver injury, attenuated the levels of oxidants (P = 0.03) and inflammatory mediators, and reduced apoptosis (P = 0). ZnO10 was found to have a greater effect on ischemic reperfusion injury than ZnO5 did. Histopathological examination also showed a dose-dependent decrease in alterations in the IR+ZnO5 and IR+ZnO10 groups.

Conclusion: Administration of ZnO5 and ZnO10 improved liver function after IR. The findings of this study suggest that ZnO NPs have a protective effect against oxidative stress and apoptosis in liver ischemia-reperfusion injury in rats. These results may have important implications for developing advanced methods in ischemia-reperfusion treatment.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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