槲寄生胆汁水提取物对肺癌、胃癌和食管癌细胞系的细胞增殖、凋亡及 CCND1、TP53、BCL2 和 BAX 基因表达的影响研究

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Pouya Tofigh, Seyed Mehdi Mirghazanfari, Zahra Hami, Ehsan Nassireslami, Mohsen Ebrahimi
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引用次数: 0

摘要

背景:槲寄生胆(QI)的治疗潜力众所周知,包括其抗炎、抗氧化和抗癌特性。然而,它对肺癌、胃癌和食道癌细胞的影响仍不清楚。本研究旨在探讨枸杞子水提取物对 A549、BGC823 和 KYSE-30 细胞系的细胞活力、凋亡和基因表达的影响:方法:将 A549、BGC823 和 KYSE-30 细胞播种在完全培养基中,然后用不同浓度的杞子提取物培养 24 小时。细胞活力通过 MTT[3-(4, 5-二甲基噻唑-2-基)-2, 5-二苯基溴化四氮唑]检测法进行测定。加入 FITC 结合物 Annexin V(Annexin V-FITC)和碘化丙啶(PI)后,通过流式细胞分析评估凋亡的诱导情况。采用实时定量聚合酶链反应分析法测定 CCND1、TP53、BCL2 和 BAX 基因的 mRNA 表达水平:MTT 试验表明,用 QI 没食子提取物处理 A549、BGC823 和 KYSE-30 细胞系后,可存活细胞数明显减少,IC50 浓度分别为 440.1、437.1 和 465.2 毫克/毫升。此外,与未处理的细胞群相比,用五倍子提取物处理 A549、BGC823 和 KYSE-30 细胞后,其早期凋亡、晚期凋亡和坏死的百分比显著增加(P< 0.05)。此外,杞胆提取物还影响了 CCND1、TP53、BCL2 和 BAX 基因的表达:本研究结果表明,祁红没食子提取物可通过诱导细胞凋亡抑制 A549、BGC823 和 KYSE-30 细胞的生长,而细胞凋亡可能是通过线粒体依赖性途径介导的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Investigation of Quercus Infectoria Gall Aqueous Extract Effect on the Cell Proliferation, Apoptosis and Expression of CCND1, TP53, BCL2 and BAX Genes in Cell Line of Lung, Gastric and Esophageal Cancers.

Background: The therapeutic potential of Quercus infectoria (QI) gall, including its anti-inflammatory, antioxidant, and anticancer properties, is well-known. However, its impact on lung, gastric, and esophageal cancer cells remain unclear. This study aims to explore the effects of QI gall aqueous extract on cell viability, apoptosis, and gene expression in A549, BGC823, and KYSE-30 cell lines.

Methods: A549, BGC823, and KYSE-30 cells were seeded in complete medium and incubated with different concentrations of QI gall extract for 24 hours. Cell viability was measured by an MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay. The induction of apoptosis was assessed through flow cytometric analysis after the adding FITC-conjugated Annexin V (Annexin V-FITC) and propidium iodide (PI). The mRNA expression levels of CCND1, TP53, BCL2 and BAX genes were determined using Real-time Quantitative Polymerase Chain Reaction analysis.

Results: The MTT assay demonstrated that treatment with QI gall extract significantly reduced the number of viable cells in the A549, BGC823, and KYSE-30 cell lines at IC50 concentrations of 440.1, 437.1, and 465.2 mg/ml, respectively. Additionally, compared to untreated cell population, the percentages of early apoptosis, late apoptosis, and necrosis in the A549, BGC823, and KYSE-30 cells significantly increased following treatment with QI gall extract (P< 0.05). Also, the treatment with QI gall extract influenced the expression of CCND1, TP53, BCL2 and BAX genes.

Conclusions: The present findings indicated that the gall extract of QI can inhibit the growth of A549, BGC823, and KYSE-30 cells by inducing apoptosis, which may be mediated via mitochondria-dependent pathway.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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