火疫病病原体 Erwinia amylovora 在苹果花柱头上增殖或在果实中致病并不需要合成天冬氨酸和酪氨酸。

IF 3.2 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Neil P Schultes, Judith P Sinn, Eric S Swenson, Timothy W McNellis
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引用次数: 0

摘要

目的:革兰氏阴性菌 Erwinia amylovora(Ea)是苹果和梨毁灭性病害火疫病的病原菌。在火疫病的病害循环中,Ea 在不同的植物组织中生长,每个组织都有不同的营养环境。在此,我们研究了天门冬氨酸和酪氨酸双辅助Ea品系在代表Ea附生生长阶段的苹果花柱头表面和代表Ea内生生长阶段的发育中果实中的增殖能力:在大肠杆菌天冬氨酸和酪氨酸辅助营养体中进行的异源互补研究证实,EaAspC 和 EaTyrB 起着天冬氨酸和酪氨酸氨基转移酶的作用。生长分析表明,Ea aspC tyrB 突变体在苹果花柱头和未成熟果实上的繁殖接近野生型水平:结论:Ea aspC 和 tyrB 在大肠杆菌和 Ea 中对天冬氨酸和酪氨酸的合成具有互补作用。Ea aspC 和 tyrB 突变体可获得足够的天门冬氨酸和酪氨酸,以支持柱头表面的繁殖和未成熟果实的毒力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synthesis of aspartic acid and tyrosine by the fire blight pathogen Erwinia amylovora is not required for proliferation on apple flower stigmas or virulence in fruitlets.

Aims: The Gram-negative bacterium Erwinia amylovora (Ea) is the causal agent of fire blight, a devastating disease of apples and pears. In the fire blight disease cycle, Ea grows in different plant tissues, each presenting a distinct nutrient environment. Here, we investigate the ability of aspartate and tyrosine double auxotroph Ea lines to proliferate on apple flower stigma surfaces representing the epiphytic growth stage of Ea and in developing fruitlets representing one endophytic growth stage of Ea.

Methods and results: Heterologous complementation studies in an Escherichia coli aspartate and tyrosine auxotroph verify that Ea aspartate aminotransferase (AspC) and tyrosine aminotransferase (TyrB) act as aspartate and tyrosine amino transferases. Growth analysis reveals that Ea aspC tyrB mutants multiply to near-wild-type levels on apple flower stigmas and immature fruitlets.

Conclusions: Ea AspC and TyrB are reciprocally complementing for aspartate and tyrosine synthesis in Ec and in Ea. Ea aspC  and  tyrB mutants obtain sufficient aspartate and tyrosine to support multiplication on stigma surfaces and virulence in immature fruitlets.

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来源期刊
Journal of Applied Microbiology
Journal of Applied Microbiology 生物-生物工程与应用微生物
CiteScore
7.30
自引率
2.50%
发文量
427
审稿时长
2.7 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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