抗生素耐药性大肠埃希菌病原菌在肠道的定植会导致溃疡性结肠炎的病情加重。

IF 4.9 2区 医学 Q1 INFECTIOUS DISEASES
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引用次数: 0

摘要

目的:大肠埃希氏菌是人类肠道中的革兰氏阴性共生菌。方法:使用元基因组学方法评估人体肠道微生物群组成和元肠道抗药性组。方法:使用元基因组学方法评估了人类肠道微生物群的组成和元肠道抗药性组,研究了大肠杆菌分离物对不同种类抗生素的敏感性。此外,还对分离出的大肠杆菌进行了基因组序列分析,以深入了解抗菌素耐药性(AMR)机制和毒力因子。研究人员还检测了 UC 和非 UC 患者的肠道蛋白质组,以了解耐药菌对宿主生理机能的影响:结果:与健康对照组(HC)相比,发现 UC 患者的元肠道抗药性基因组(829 个)以 AMR 基因为主[518]。元基因组研究显示,与城市(健康对照组为 340 个;慢性阻塞性肺病患者为 578 个)相比,农村人口(健康对照组为 378 个;慢性阻塞性肺病患者为 607 个)的 AMR 基因流行率更高。在所有大肠杆菌分离物中,约有 40% 的大肠杆菌具有多重耐药性,与 HC(33.33%)相比,UC(43.75%)的流行率更高。抗菌人类蛋白(乳转铁蛋白、氮芥苷、嗜蛋白酶 G、中性粒细胞弹性蛋白酶和中性粒细胞防御素 3)和免疫蛋白(蛋白 S100-A9 和蛋白 S100-A8)的表达上调表明 UC 肠道中存在微生物感染:结论:除了传统的培养组学方法外,多组学策略还能让人更深入地了解 UC 患者的病因、病原菌的出现及其在破坏健康肠道环境中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Gut Colonization With Antibiotic-Resistant Escherichia coli Pathobionts Leads to Disease Severity in Ulcerative Colitis

Gut Colonization With Antibiotic-Resistant Escherichia coli Pathobionts Leads to Disease Severity in Ulcerative Colitis

Background

Escherichia coli is a Gram-negative commensal of human gut. Surprisingly, the role of E. coli in the pathogenesis of ulcerative colitis (UC) has not been explored until now.

Methods

Human gut microbiota composition and meta-gut resistome were evaluated using metagenomics. Antibiotic susceptibility of E. coli isolates against different class of antibiotics was investigated. Further, the genome sequence analysis of E. coli isolates was performed to gain insight into the antimicrobial resistance (AMR) mechanism and virulence factors. Gut proteome of UC and non-UC was examined to understand the effect of resistant bacteria on host physiology.

Results

In UC patients, meta-gut resistome was found to be dominated by AMR genes (829) compared to healthy controls (HC) [518]. The metagenome study revealed a higher prevalence of AMR genes in the rural population (378 in HC; 607 in UC) compared to the urban (340 in HC; 578 in UC). Approximately, 40% of all E. coli isolates were multi-drug resistant (MDR), with higher prevalence in UC (43.75%) compared to HC (33.33%). Up-regulated expression of antimicrobial human proteins (lactotransferrin, azurocidin, cathepsin G, neutrophil elastase, and neutrophil defensin 3) and inflammatory mediator (Protein S100-A9 and Protein S100-A8) suggest microbial infection in UC gut.

Conclusions

In addition to the conventional culturomics method, a multi-omics strategy provides deeper insights into the disease etiology, emergence of MDR pathobionts, and their roles in the disruption of the healthy gut environment in UC patients.

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来源期刊
CiteScore
21.60
自引率
0.90%
发文量
176
审稿时长
36 days
期刊介绍: The International Journal of Antimicrobial Agents is a peer-reviewed publication offering comprehensive and current reference information on the physical, pharmacological, in vitro, and clinical properties of individual antimicrobial agents, covering antiviral, antiparasitic, antibacterial, and antifungal agents. The journal not only communicates new trends and developments through authoritative review articles but also addresses the critical issue of antimicrobial resistance, both in hospital and community settings. Published content includes solicited reviews by leading experts and high-quality original research papers in the specified fields.
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