PpBBX32 和 PpZAT5 调节桃果中花青素的温度依赖性和组织特异性积累

IF 8.7 1区 农林科学 Q1 Agricultural and Biological Sciences
Dan Huang, Lei Xue, Yueqin Lu, Mengfei Liu, Kui Lin-Wang, Andrew C Allan, Bo Zhang, Kunsong Chen, Changjie Xu
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引用次数: 0

摘要

花青素是影响果实品质和营养的重要化合物。众所周知,R2R3 MYB 转录因子 PpMYB10.1 对调节桃中花青素的积累至关重要。然而,PpMYB10.1 上游控制温度依赖性、栽培品种对比性和组织特异性花青素积累的调控因子仍有待确定。本研究观察了桃[Prunus persica (L.) Batsch]栽培品种 "中华寿桃 "和 "冬雪蜜 "之间,以及 "中华寿桃 "不同贮藏温度和不同果实组织之间近果皮外层果肉(OF)花青素积累的差异。通过交叉比较不同花青素积累样本的 RNA-Seq 数据,发现了转录因子基因 PpBBX32 和 PpZAT5。通过瞬时表达和遗传转化,这两个基因被鉴定为花青素积累的两个正向调节因子。各种相互作用试验表明,PpBBX32 和 PpZAT5 可直接激活 PpMYB10.1 启动子,同时在蛋白质水平上相互作用,形成 PpZAT5-PpBBX32-PpMYB10.1 复合物。此外,硅学分析和外源施加茉莉酸甲酯(MeJA)的结果表明,MeJA 有利于花青素的积累,同时还发现花青素的积累以及 PpBBX32 和 PpZAT5 的表达与不同果实组织中的内源 JA 和 JA-Ile 显著相关。总之,PpBBX32 和 PpZAT5 是 PpMYB10.1 的上游激活剂,通过调节它们的表达,JA 可以参与温度依赖性和组织特异性的花青素积累。这项工作丰富了人们对内外因素作用下不同花青素积累的转录调控机制的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PpBBX32 and PpZAT5 modulate temperature-dependent and tissue-specific anthocyanin accumulation in peach fruit
Anthocyanins are important compounds for fruit quality and nutrition. The R2R3 MYB transcription factor PpMYB10.1 is known to be critical for regulating anthocyanin accumulation in peach. However, regulatory factors upstream of PpMYB10.1 which control temperature-dependent, cultivar-contrasted and tissue-specific anthocyanin accumulation remain to be determined. In this study, differential anthocyanin accumulation in the outer flesh near the peel (OF) of peach [Prunus persica (L.) Batsch] was observed between cultivars “Zhonghuashoutao” and “Dongxuemi”, as well as among different storage temperatures and different fruit tissues of “Zhonghuashoutao”. By cross-comparisons of RNA-Seq data of samples with differential anthocyanin accumulation, transcription factor genes PpBBX32 and PpZAT5 were identified. These were functionally characterized as two positive regulators for anthocyanin accumulation via transient expression and genetic transformation. Various interaction assays revealed that both PpBBX32 and PpZAT5 can directly activate the PpMYB10.1 promoter and meanwhile interact at protein level as a PpZAT5-PpBBX32-PpMYB10.1 complex. Furthermore, the results of in silico analysis and exogenous application of methyl jasmonate (MeJA) indicated that MeJA favored anthocyanin accumulation, while it was also found that anthocyanin accumulation as well as PpBBX32 and PpZAT5 expression correlated significantly with endogenous JA and JA-Ile in different fruit tissues. In summary, PpBBX32 and PpZAT5 are upstream activators of PpMYB10.1, allowing JAs to take part in temperature-dependent and tissue-specific anthocyanin accumulation by modulating their expression. This work enriches the knowledge of the transcriptional regulatory mechanisms for differential anthocyanin accumulation under internal and external factors.
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来源期刊
Horticulture Research
Horticulture Research Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
11.20
自引率
6.90%
发文量
367
审稿时长
20 weeks
期刊介绍: Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.
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