番茄萎黄病病毒对粉虱媒介和寄主植物的双重影响促进病毒传播

IF 4.3 1区 农林科学 Q1 ENTOMOLOGY
Jicheng Wang, Ying Liu, Yuxin Zhang, Penghao Qin, Jie Li, Jixing Xia, Youjun Zhang, Dong Chu
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引用次数: 0

摘要

番茄萎黄病病毒(ToCV)在全球番茄中广泛传播,并在中国迅速蔓延,它与优势粉虱地中海粉虱(Bemisia tabaci (Gennadius) Mediterranean (MED))密切相关。据报道,带病毒的粉虱偏爱健康的番茄植株,从而极大地促进了该病毒的传播。然而,宿主选择行为发生变化的机制尚不清楚。我们研究了 ToCV 感染对番茄植株挥发物排放的影响,以确定与 B. tabaci MED 的宿主选择性相关的主要挥发物。气相色谱质谱(GC-MS)分析表明,ToCV 感染显著改变了 11 种挥发性化合物的浓度。值得注意的是,具有吸引力的对乙基苯乙酮减少了,而具有排斥性的丁基羟基甲苯增加了。RT-qPCR 显示,与不带毒的粉虱相比,带毒粉虱的 10 个气味结合蛋白(OBP)基因和 11 个化感蛋白(CSP)基因的表达发生了显著变化。RNA 干扰表明,沉默 OBP-17 或 CSP-1 会引发烟粉虱对西红柿的强烈排斥反应。此外,OBP-17 的表达量随着对乙基苯乙酮的暴露而显著增加,OBP-17 和 CSP-1 与这种化合物的结合亲和力很强,其解离常数分别为 KD OBP-17 = 17.24 µmol/L 和 KD CSP-1 = 15.02 µmol/L。总之,我们的研究揭示了 ToCV 对病媒粉虱及其寄主植物的双重作用,这两种作用共同促进了病毒的传播。对昆虫传播的植物病毒的流行病学机制的这一新颖见解可能有助于开发控制这些极其重要的农业害虫的新策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Dual effects of tomato chlorosis virus on its whitefly vector and its host plant to facilitate viral spread

Dual effects of tomato chlorosis virus on its whitefly vector and its host plant to facilitate viral spread

Tomato chlorosis virus (ToCV) causes widespread infections in tomatoes globally, rapidly spreading in China is closely associated with the dominant whitefly, Bemisia tabaci (Gennadius) Mediterranean (MED). Viruliferous whiteflies have been reported to have shown preference for healthy tomato plants and thus greatly facilitate the spread of this virus. However, the mechanism underlying the change in the host selection behavior is yet unknown. We studied the effects of ToCV infection on the volatile emissions of tomato plants to determine the main volatiles associated with host selectivity by B. tabaci MED. Gas chromatography mass spectrometry (GC-MS) analyses revealed that ToCV infection significantly altered concentrations of 11 volatile compounds. Notably, the attractive p-ethylacetophenone, decreased, while the repellent butylated hydroxytoluene increased. RT-qPCR showed significant expression changes in 10 odorant binding protein (OBP) genes and 11 chemosensory protein (CSP) genes in viruliferous whiteflies compared to non-viruliferous whiteflies. RNA interference indicated that silencing OBP-17 or CSP-1 triggered a strong repellent response from B. tabaci toward tomatoes. Furthermore, OBP-17 expression rose notably with p-ethylacetophenone exposure, with both OBP-17 and CSP-1 exhibiting strong binding affinity for this compound, having dissociation constants of KD OBP-17 = 17.24 µmol/L and KD CSP-1 = 15.02 µmol/L, respectively. In conclusion, our study revealed dual effects of ToCV on its vectoring whitefly as well as its host plant, which together facilitate the spread of the virus. This novel insight into the epidemiological mechanisms of insect-vectored plant viruses may help to develop new strategies to control these exceedingly important agricultural pests.

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来源期刊
Journal of Pest Science
Journal of Pest Science 生物-昆虫学
CiteScore
10.40
自引率
8.30%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues. Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates. Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management. Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.
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