在女性不孕症患者中检测衣原体热休克蛋白 60 和 10 抗体

IF 1 Q4 INFECTIOUS DISEASES
Journal of Global Infectious Diseases Pub Date : 2024-06-26 eCollection Date: 2024-04-01 DOI:10.4103/jgid.jgid_147_23
Gopi Dhivya, Kopula Sathyamoorthy Sridharan, Sanjeeva Reddy Nellepalli, P Kennedy Kumar, Arunagiri Ramesh, Divya Katta
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引用次数: 0

摘要

导言:在众多性传播病原体中,沙眼衣原体除了引起生殖道感染外,还越来越多地与不孕症等长期后遗症相关联。许多针对衣原体感染的炎症反应可造成输卵管损伤,导致不孕。例如,衣原体热休克蛋白 60(cHSP60)和 cHSP10 以及体液免疫反应。我们的研究旨在检测女性不孕症患者体内是否存在针对主要外膜蛋白(MOMP)、cHSP60 和 cHSP10 的免疫球蛋白 G(IgG)抗体:研究共纳入了 230 名在 SRIHER 生殖医学门诊部就诊的女性不孕症患者。在表格中详细记录病史。通过酶联免疫吸附试验(ELISA)检测沙眼衣原体 IgG 抗体对 MOMP、cHSP60 和 cHSP10 的抗体:结果:230 名女性中有 15 人(6.5%)检测到沙眼衣原体 MOMP IgG 抗体。在输卵管因素不孕(TFI)的女性中,cHSP60抗体的血清阳性率很高。我们的研究显示,cHSP60抗体(3.4%)比cHSP10(2.6%)更常见:我们的研究表明,通过 ELISA 检测 cHSP60 或 cHSP10 抗体以及 TFI 有助于诊断和早期治疗。与原发性不孕症相比,在继发性不孕症中检测抗MOMP和cHSP60抗体可提高TFI预测的准确性。继发性不孕症患者血清阳性率高的最大原因可能是反复感染和性生活活跃时间较长导致的慢性感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of Chlamydial Heat Shock Protein 60 and 10 Antibody among Female Infertility.

Introduction: Of the many sexually transmitted pathogens, Chlamydia trachomatis is increasingly being associated with long-term sequelae such as infertility, apart from causing genital tract infections. Many inflammatory responses directed against chlamydial infection can cause tubal damage resulting in infertility. For example, chlamydial heat shock protein 60 (cHSP60) and cHSP10 along with humoral immune response. The aim of our study is to detect the presence of immunoglobulin G (IgG) antibodies against Major Outer Membrane Protein (MOMP), cHSP60, and cHSP10 among female infertility.

Methods: A total number of 230 female infertility patients attending the Outpatient Department of Reproductive Medicine, SRIHER, were included in the study. Detailed history documented in the proforma. Serological detection of C. trachomatis IgG antibody against MOMP, cHSP60, and cHSP10 antibody was done by enzyme-linked immunosorbent assay (ELISA).

Results: C. trachomatis IgG antibody against MOMP was detected in 15 (6.5%) of 230 females. High seropositivity to cHSP60 antibodies was detected among females of tubal factor infertility (TFI). Our study showed that cHSP60 antibodies (3.4%) were more common than cHSP10 (2.6%).

Conclusion: Our study suggest cHSP60 or cHSP10 antibody detection by ELISA along with TFI is helpful for diagnosis and early institution of therapy. The accuracy of TFI prediction could be increased by the detection of anti-MOMP and cHSP60 over cHSP10 among secondary infertility than primary. The most probable reason for high seropositivity among secondary infertility patients may be due to repeated infection and chronicity because of longer active sexual life.

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来源期刊
CiteScore
3.30
自引率
0.00%
发文量
31
审稿时长
29 weeks
期刊介绍: JGID encourages research, education and dissemination of knowledge in the field of Infectious Diseases across the world thus promoting translational research by striking a synergy between basic science, clinical medicine and public health. The Journal intends to bring together scientists and academicians in Infectious Diseases to promote translational synergy between Laboratory Science, Clinical Medicine and Public Health. The Journal invites Original Articles, Clinical Investigations, Epidemiological Analysis, Data Protocols, Case Reports, Clinical Photographs, review articles and special commentaries. Students, Residents, Academicians, Public Health experts and scientists are all encouraged to be a part of this initiative by contributing, reviewing and promoting scientific works and science.
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