{"title":"Desertibaculum subflavum gen.","authors":"Chu-Ying Feng, Huan-Huan He, Shuai Li, Zhuo-Huan Zheng, Yi-Jun Mo, Wen-Hui Lian, Chun-Yan Lu, Dong-Ya Zhang, Wen-Jun Li, Lei Dong","doi":"10.1007/s10482-024-02003-6","DOIUrl":null,"url":null,"abstract":"<div><p>A novel rod-shaped bacterium, designated as strain SYSU D60015<sup>T</sup> that formed yellowish colonies was isolated from a sandy soil collected from the Kumtag Desert in Xinjiang, China. Cells were Gram-stain-negative, oxidase-positive, catalase-negative and motile with a single polar flagellum. Growth optimum occurred between 28 and 37 °C, pH 7.0 and with 0–0.5% (W/V) NaCl. The predominant cellular fatty acids (> 5%) were summed feature 8 (C<sub>18:1</sub> <i>ω</i>7<i>c</i> and/or C<sub>18:1</sub> <i>ω</i>6<i>c</i>), C<sub>19:0</sub> cyclo <i>ω</i>8<i>c</i>, C<sub>18:1</sub> <i>ω</i>7<i>c</i> 11-methyl and C<sub>16:0</sub>. The polar lipid profile contained one phosphatidylethanolamine, one diphosphatidylglycerol, one phosphatidylglycerol, one unidentified phospholipid, three unidentified aminolipids, two unidentified aminophospholipids and seven unidentified lipids. The only respiratory quinone was ubiquinone-10. Based on 16S rRNA gene sequence phylogenetic analysis, strain SYSU D60015<sup>T</sup> was found to form a distinct linage within the family <i>Sneathiellaceae</i>, and had 16S rRNA gene sequence similarities of 90.8% to <i>Taonella mepensis</i> H1<sup>T</sup>, and 90.2% to <i>Ferrovibrio denitrificans</i> S3<sup>T</sup>. The genome of SYSU D60015<sup>T</sup> was 5.66 Mb in size with 68.2% of DNA G + C content. The low digital DNA-DNA hybridization (dDDH, 18.0%), average nucleotide identity (ANI, 77.5%) and amino acid identity (AAI, 56.0%) values between SYSU D60015<sup>T</sup> and <i>Ferrovibrio terrae</i> K5<sup>T</sup> indicated that SYSU D60015<sup>T</sup> might represent a distinct genus. Based on the phylogenetic, phenotypic, chemotaxonomic and genomic data, we propose <i>Desertibaculum subflavum</i> gen. nov., sp. nov. as a novel species of a new genus within the family <i>Sneathiellaceae</i>. The type strain is SYSU D60015<sup>T</sup> (= NBRC 112952<sup>T</sup> = CGMCC 1.16256<sup>T</sup>).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Desertibaculum subflavum gen. nov., sp. nov., a novel member of the family Sneathiellaceae isolated from the Kumtag Desert soil\",\"authors\":\"Chu-Ying Feng, Huan-Huan He, Shuai Li, Zhuo-Huan Zheng, Yi-Jun Mo, Wen-Hui Lian, Chun-Yan Lu, Dong-Ya Zhang, Wen-Jun Li, Lei Dong\",\"doi\":\"10.1007/s10482-024-02003-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A novel rod-shaped bacterium, designated as strain SYSU D60015<sup>T</sup> that formed yellowish colonies was isolated from a sandy soil collected from the Kumtag Desert in Xinjiang, China. Cells were Gram-stain-negative, oxidase-positive, catalase-negative and motile with a single polar flagellum. Growth optimum occurred between 28 and 37 °C, pH 7.0 and with 0–0.5% (W/V) NaCl. The predominant cellular fatty acids (> 5%) were summed feature 8 (C<sub>18:1</sub> <i>ω</i>7<i>c</i> and/or C<sub>18:1</sub> <i>ω</i>6<i>c</i>), C<sub>19:0</sub> cyclo <i>ω</i>8<i>c</i>, C<sub>18:1</sub> <i>ω</i>7<i>c</i> 11-methyl and C<sub>16:0</sub>. The polar lipid profile contained one phosphatidylethanolamine, one diphosphatidylglycerol, one phosphatidylglycerol, one unidentified phospholipid, three unidentified aminolipids, two unidentified aminophospholipids and seven unidentified lipids. The only respiratory quinone was ubiquinone-10. Based on 16S rRNA gene sequence phylogenetic analysis, strain SYSU D60015<sup>T</sup> was found to form a distinct linage within the family <i>Sneathiellaceae</i>, and had 16S rRNA gene sequence similarities of 90.8% to <i>Taonella mepensis</i> H1<sup>T</sup>, and 90.2% to <i>Ferrovibrio denitrificans</i> S3<sup>T</sup>. The genome of SYSU D60015<sup>T</sup> was 5.66 Mb in size with 68.2% of DNA G + C content. The low digital DNA-DNA hybridization (dDDH, 18.0%), average nucleotide identity (ANI, 77.5%) and amino acid identity (AAI, 56.0%) values between SYSU D60015<sup>T</sup> and <i>Ferrovibrio terrae</i> K5<sup>T</sup> indicated that SYSU D60015<sup>T</sup> might represent a distinct genus. Based on the phylogenetic, phenotypic, chemotaxonomic and genomic data, we propose <i>Desertibaculum subflavum</i> gen. nov., sp. nov. as a novel species of a new genus within the family <i>Sneathiellaceae</i>. The type strain is SYSU D60015<sup>T</sup> (= NBRC 112952<sup>T</sup> = CGMCC 1.16256<sup>T</sup>).</p></div>\",\"PeriodicalId\":50746,\"journal\":{\"name\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"volume\":\"117 1\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-07-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10482-024-02003-6\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10482-024-02003-6","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Desertibaculum subflavum gen. nov., sp. nov., a novel member of the family Sneathiellaceae isolated from the Kumtag Desert soil
A novel rod-shaped bacterium, designated as strain SYSU D60015T that formed yellowish colonies was isolated from a sandy soil collected from the Kumtag Desert in Xinjiang, China. Cells were Gram-stain-negative, oxidase-positive, catalase-negative and motile with a single polar flagellum. Growth optimum occurred between 28 and 37 °C, pH 7.0 and with 0–0.5% (W/V) NaCl. The predominant cellular fatty acids (> 5%) were summed feature 8 (C18:1ω7c and/or C18:1ω6c), C19:0 cyclo ω8c, C18:1ω7c 11-methyl and C16:0. The polar lipid profile contained one phosphatidylethanolamine, one diphosphatidylglycerol, one phosphatidylglycerol, one unidentified phospholipid, three unidentified aminolipids, two unidentified aminophospholipids and seven unidentified lipids. The only respiratory quinone was ubiquinone-10. Based on 16S rRNA gene sequence phylogenetic analysis, strain SYSU D60015T was found to form a distinct linage within the family Sneathiellaceae, and had 16S rRNA gene sequence similarities of 90.8% to Taonella mepensis H1T, and 90.2% to Ferrovibrio denitrificans S3T. The genome of SYSU D60015T was 5.66 Mb in size with 68.2% of DNA G + C content. The low digital DNA-DNA hybridization (dDDH, 18.0%), average nucleotide identity (ANI, 77.5%) and amino acid identity (AAI, 56.0%) values between SYSU D60015T and Ferrovibrio terrae K5T indicated that SYSU D60015T might represent a distinct genus. Based on the phylogenetic, phenotypic, chemotaxonomic and genomic data, we propose Desertibaculum subflavum gen. nov., sp. nov. as a novel species of a new genus within the family Sneathiellaceae. The type strain is SYSU D60015T (= NBRC 112952T = CGMCC 1.16256T).
期刊介绍:
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.