LGG 通过 TLR2 受体促进肠道 ILC3 的活化,抑制小鼠鼠伤寒沙门氏菌感染。

IF 5.5 1区 农林科学 Q1 IMMUNOLOGY
Virulence Pub Date : 2024-12-01 Epub Date: 2024-07-30 DOI:10.1080/21505594.2024.2384553
Junhong Wang, Ming Gao, Jiarui Wang, Yan Zeng, Chunfeng Wang, Xin Cao
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引用次数: 0

摘要

沙门氏菌是一种食源性病原体,会破坏肠道粘膜免疫,导致宿主急性肠胃炎。在这项研究中,我们发现鼠伤寒沙门氏菌(STM)感染小鼠肠道后,乳酸杆菌的比例显著增加,而 3 型先天性淋巴细胞(ILC3)分泌的 IL-22 显著增加。喂食鼠李糖乳杆菌 GG(LGG)能有效缓解小鼠肠道中的 STM 感染。TLR2-/-小鼠实验发现,表达 TLR2 的树突状细胞(DCs)对 LGG 激活 ILC3 至关重要。随后的体外实验表明,热处理后的LGG(HK-LGG)能促进DCs分泌IL-23,从而进一步促进ILC3的活化和IL-22的分泌。最后,类器官实验进一步验证了 ILC3 分泌的 IL-22 可增强肠粘膜免疫屏障,抑制 STM 感染。这项研究表明,口服 LGG 是一种潜在的抑制 STM 感染的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
LGG promotes activation of intestinal ILC3 through TLR2 receptor and inhibits salmonella typhimurium infection in mice.

Salmonella is a foodborne pathogen that causes disruption of intestinal mucosal immunity, leading to acute gastroenteritis in the host. In this study, we found that Salmonella Typhimurium (STM) infection of the intestinal tract of mice led to a significant increase in the proportion of Lacticaseibacillus, while the secretion of IL-22 from type 3 innate lymphoid cells (ILC3) increased significantly. Feeding Lacticaseibacillus rhamnosus GG (LGG) effectively alleviated the infection of STM in the mouse intestines. TLR2-/- mice experiments found that TLR2-expressing dendritic cells (DCs) are crucial for LGG's activation of ILC3. Subsequent in vitro experiments showed that heat-killed LGG (HK-LGG) could promote DCs to secrete IL-23, which in turn further promotes the activation of ILC3 and the secretion of IL-22. Finally, organoid experiments further verified that IL-22 secreted by ILC3 can enhance the intestinal mucosal immune barrier and inhibit STM infection. This study demonstrates that oral administration of LGG is a potential method for inhibiting STM infection.

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来源期刊
Virulence
Virulence IMMUNOLOGY-MICROBIOLOGY
CiteScore
9.20
自引率
1.90%
发文量
123
审稿时长
6-12 weeks
期刊介绍: Virulence is a fully open access peer-reviewed journal. All articles will (if accepted) be available for anyone to read anywhere, at any time immediately on publication. Virulence is the first international peer-reviewed journal of its kind to focus exclusively on microbial pathogenicity, the infection process and host-pathogen interactions. To address the new infectious challenges, emerging infectious agents and antimicrobial resistance, there is a clear need for interdisciplinary research.
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