利用生物信息学鉴定皮肌炎和鼻咽癌的共同免疫浸润特征分子:皮肌炎和鼻咽癌的特征。

IF 2 4区 医学 Q3 DERMATOLOGY
Jinyan Kai, Haitao Huang, Jiaqi Su, Qiong Chen
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引用次数: 0

摘要

背景:皮肌炎(Dermatomyositis,DM)是一种与皮肤相关的自身免疫性疾病,与并发恶性肿瘤的风险增加显著相关,但其潜在机制仍有待完全阐明。本研究的目的是研究DM与鼻咽癌(NPC)之间的免疫学相似性,旨在确定关键的生物标志物,以促进对DM患者鼻咽癌的深入了解并提高其预测能力:方法:DM和鼻咽癌的数据来自基因表达总库(GEO)数据库。使用 "cibersort "R软件包分析免疫浸润,使用 "limma "软件包确定差异表达基因(DEG),并通过京都基因组百科全书(KEGG)和基因本体(GO)分析研究功能通路。利用蛋白质-蛋白质相互作用(PPI)和最小绝对收缩与选择操作器(LASSO)确定了特征基因,并利用GSE53819数据集对其特征进行了验证:与正常样本相比,在DM和鼻咽癌中都观察到了巨噬细胞M1的显著浸润。分析发现,DM中有77个DEGs,NPC中有1051个DEGs,其中有22个基因是共DEGs。经过PPI和LASSO分析,保留了6个独特的基因。值得注意的是,CCL8、IFIH1、CXCL10 和 CXCL11 对鼻咽癌具有最佳诊断效果,并与癌细胞内巨噬细胞 M1 的浸润有显著相关性:结论:CCL8、IFIH1、CXCL10 和 CXCL11 这四个特征基因是 DM 和鼻咽癌的风险因素。它们与鼻咽癌的发病率密切相关,具有值得称道的诊断功效。此外,它们还可作为 DM 中出现鼻咽癌的前瞻性预测生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of shared immune infiltration characteristic molecules in dermatomyositis and nasopharyngeal carcinoma using bioinformatics: Traits in dermatomyositis and nasopharyngeal cancer.

Background: Dermatomyositis (DM) is a kind of dermatologically associated autoimmune disease that is notably associated with an increased risk of concurrent malignancies, although the underlying mechanisms remain to be fully elucidated. The purpose of this investigation was to examine the immunological parallels between DM and nasopharyngeal carcinoma (NPC), with the aim of identifying pivotal biomarkers that could facilitate a deeper understanding and enhance the predictive capabilities of NPC in DM patients.

Method: Data for DM and NPC were sourced from the Gene Expression Omnibus (GEO) database. Immune infiltration was analyzed using the "cibersort" R package, differentially expressed genes (DEGs) were identified with the "limma" package, and functional pathways were investigated through Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses. Characteristic genes were determined by Utilizing Protein-Protein Interaction (PPI) and Least Absolute Shrinkage and Selection Operator (LASSO), and their features were validated using the GSE53819 dataset.

Results: In comparison to normal samples, significant infiltration of macrophage M1 was observed in both DM and NPC. The analysis revealed 77 DEGs in DM and 1051 DEGs in NPC, with 22 genes found to be co-DEGs. Following PPI and LASSO analysis, six distinctive genes were retained. Notably, CCL8, IFIH1, CXCL10, and CXCL11 exhibited optimal diagnostic efficacy for NPC and displayed significant correlation with macrophage M1 infiltration within the carcinoma.

Conclusion: Four characteristic genes, CCL8, IFIH1, CXCL10, and CXCL11 are risk factors for both DM and NPC. They exhibit a robust correlation with the incidence of NPC and offer a commendable diagnostic efficacy. Furthermore, they may serve as prospective predictive biomarkers for the emergence of NPC in DM.

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来源期刊
Skin Research and Technology
Skin Research and Technology 医学-皮肤病学
CiteScore
3.30
自引率
9.10%
发文量
95
审稿时长
6-12 weeks
期刊介绍: Skin Research and Technology is a clinically-oriented journal on biophysical methods and imaging techniques and how they are used in dermatology, cosmetology and plastic surgery for noninvasive quantification of skin structure and functions. Papers are invited on the development and validation of methods and their application in the characterization of diseased, abnormal and normal skin. Topics include blood flow, colorimetry, thermography, evaporimetry, epidermal humidity, desquamation, profilometry, skin mechanics, epiluminiscence microscopy, high-frequency ultrasonography, confocal microscopy, digital imaging, image analysis and computerized evaluation and magnetic resonance. Noninvasive biochemical methods (such as lipids, keratin and tissue water) and the instrumental evaluation of cytological and histological samples are also covered. The journal has a wide scope and aims to link scientists, clinical researchers and technicians through original articles, communications, editorials and commentaries, letters, reviews, announcements and news. Contributions should be clear, experimentally sound and novel.
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