YTHDF1（而非YTHDF3）的缺失可减少脑外伤后大脑和肠道的损伤。

IF 1.7 4区医学 Q3 CLINICAL NEUROLOGY

Neurological Research Pub Date : 2024-11-01 Epub Date: 2024-07-30 DOI:10.1080/01616412.2024.2381160

Wei Zhao, Ruzhi Li, Xiang Zhong, Peizan Huang

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引用次数: 0

摘要

目的确定YTHDF1和YTHDF3在创伤性脑损伤（TBI）后脑损伤和肠道损伤中是否发挥相同的作用：方法：利用CRISPR/Cas9技术产生YTHDF1-/-和YTHDF3-/-小鼠，通过严重的可控皮层冲击（CCI）建立小鼠脑损伤模型，通过分析CCI后3天小鼠皮层组织水肿蛋白和脑肠组织炎症蛋白水平及组织病理学病变，观察YTHDF1-/-和YTHDF3-/-小鼠的不同损伤类型：结果：与WT小鼠相比，YTHDF1-/-小鼠在CCI后3天皮质组织中的水肿蛋白水平以及脑组织和肠道组织中的炎症蛋白和组织病理学病变水平均有所下降，但YTHDF3-/-小鼠没有下降：我们的研究结果表明，删除 YTHDF1（而非 YTHDF3）可减少创伤性脑损伤后对大脑和肠道的损伤。

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Deletion of YTHDF1 (not YTHDF3) reduced brain and gut damage after traumatic brain injury.

Objective: To determine whether YTHDF1 and YTHDF3 play the same role in brain and gut damage after traumatic brain injury (TBI).

Methods: We generated YTHDF1-/- and YTHDF3-/- mice using CRISPR/Cas9 technology, established a mouse brain injury model through severe controlled cortical impact (CCI), and finally observed the different types of damage between YTHDF1-/- and YTHDF3-/- mice by analysing the levels of oedema proteins in cortical tissue and inflammatory proteins and histopathological lesions in brain and gut tissues in mice at 3 days after CCI.

Result: Compared with WT mice, YTHDF1-/- mice had decreased levels of oedema in cortical tissue and inflammation and histopathological lesions in brain and gut tissues at 3 days post-CCI, but YTHDF3-/- mice did not.

Conclusion: Our results suggest that deletion of YTHDF1, but not YTHDF3, could reduce damage to the brain and gut following TBI.

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来源期刊

Neurological Research 医学-临床神经学

CiteScore

3.60

自引率

0.00%

发文量

116

审稿时长

5.3 months

期刊介绍： Neurological Research is an international, peer-reviewed journal for reporting both basic and clinical research in the fields of neurosurgery, neurology, neuroengineering and neurosciences. It provides a medium for those who recognize the wider implications of their work and who wish to be informed of the relevant experience of others in related and more distant fields. The scope of the journal includes: •Stem cell applications •Molecular neuroscience •Neuropharmacology •Neuroradiology •Neurochemistry •Biomathematical models •Endovascular neurosurgery •Innovation in neurosurgery.