Mona M M Y Elghandour, Edson Brodeli Figueroa Pacheco, Ameer Khusro, Deli Nazmín Tirado-González, Maximilian Lackner, José Luis Ponce-Covarrubias, Pasquale De Palo, Aristide Maggiolino, Abdelfattah Z M Salem
{"title":"解读辣木籽和益生菌对减轻反刍动物沼气生产的作用。","authors":"Mona M M Y Elghandour, Edson Brodeli Figueroa Pacheco, Ameer Khusro, Deli Nazmín Tirado-González, Maximilian Lackner, José Luis Ponce-Covarrubias, Pasquale De Palo, Aristide Maggiolino, Abdelfattah Z M Salem","doi":"10.1186/s13568-024-01744-x","DOIUrl":null,"url":null,"abstract":"<p><p>Maintaining cleaner and more sustainable ecosystems by mitigating greenhouse gas (GHG) emissions from livestock through dietary manipulation is in demand. This study was aimed to assess the effect of Moringa oleifera seeds and probiotics (Pediococcus acidilactici BX-B122 and Bacillus coagulans BX-B118) as feed supplements on GHG production and fermentation profile from steers and sheep. The treatments included diets containing 0, 6, 12, and 18% of M. oleifera seeds meal and a mixture of probiotic bacteria (0.2 ml/g of diet). Total biogas production, CH<sub>4</sub>, CO, and H<sub>2</sub>S emission from animals (up to 48 h), rumen fermentation profile, and CH<sub>4</sub> conversion efficiency were recorded using standard protocols. Results showed interaction among M. oleifera seeds and probiotics on asymptotic biogas production and total biogas production up to 48 h (P < 0.05). The rate of CH<sub>4</sub> emission in steers was reduced from 0.1694 to 0.0447 ml/h using 6 and 18% of M. oleifera seeds (P < 0.05). Asymptotic CO and the rate of CO production were increased (P < 0.05) by supplementing different doses of M. oleifera seeds and probiotics. Adding 12% of M. oleifera seeds and probiotics reduced H<sub>2</sub>S production from 0.0675 to 0.0112 ml H<sub>2</sub>S/g DM (at 48 h of fermentation) in steers. In sheep, the additives mitigated H<sub>2</sub>S production from 0.0364 to 0.0029 ml H<sub>2</sub>S/g DM (at 48 h of fermentation), however there were not interaction (P = 0.7744). In addition, M. oleifera seeds and probiotics reduced the pH level and dry matter degradability (DMD) in steers and sheep (P < 0.0001) showing a positive impact on CH<sub>4</sub>:ME and CH<sub>4</sub>:OM (in steers) and CH<sub>4</sub>:SCFA (in sheep), while the interaction was not significant (P > 0.05) for CH<sub>4</sub>:SCFA (in steers) and CH<sub>4</sub>:ME and CH<sub>4</sub>:OM (in sheep). In conclusion, the interaction of M. oleifera seeds and probiotics in the feeding diet reduced GHG emissions and affected the fermentation profile of steers and sheep.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11289196/pdf/","citationCount":"0","resultStr":"{\"title\":\"Deciphering the role of Moringa oleifera seeds and probiotic bacteria on mitigation of biogas production from ruminants.\",\"authors\":\"Mona M M Y Elghandour, Edson Brodeli Figueroa Pacheco, Ameer Khusro, Deli Nazmín Tirado-González, Maximilian Lackner, José Luis Ponce-Covarrubias, Pasquale De Palo, Aristide Maggiolino, Abdelfattah Z M Salem\",\"doi\":\"10.1186/s13568-024-01744-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Maintaining cleaner and more sustainable ecosystems by mitigating greenhouse gas (GHG) emissions from livestock through dietary manipulation is in demand. This study was aimed to assess the effect of Moringa oleifera seeds and probiotics (Pediococcus acidilactici BX-B122 and Bacillus coagulans BX-B118) as feed supplements on GHG production and fermentation profile from steers and sheep. The treatments included diets containing 0, 6, 12, and 18% of M. oleifera seeds meal and a mixture of probiotic bacteria (0.2 ml/g of diet). Total biogas production, CH<sub>4</sub>, CO, and H<sub>2</sub>S emission from animals (up to 48 h), rumen fermentation profile, and CH<sub>4</sub> conversion efficiency were recorded using standard protocols. Results showed interaction among M. oleifera seeds and probiotics on asymptotic biogas production and total biogas production up to 48 h (P < 0.05). The rate of CH<sub>4</sub> emission in steers was reduced from 0.1694 to 0.0447 ml/h using 6 and 18% of M. oleifera seeds (P < 0.05). Asymptotic CO and the rate of CO production were increased (P < 0.05) by supplementing different doses of M. oleifera seeds and probiotics. Adding 12% of M. oleifera seeds and probiotics reduced H<sub>2</sub>S production from 0.0675 to 0.0112 ml H<sub>2</sub>S/g DM (at 48 h of fermentation) in steers. In sheep, the additives mitigated H<sub>2</sub>S production from 0.0364 to 0.0029 ml H<sub>2</sub>S/g DM (at 48 h of fermentation), however there were not interaction (P = 0.7744). In addition, M. oleifera seeds and probiotics reduced the pH level and dry matter degradability (DMD) in steers and sheep (P < 0.0001) showing a positive impact on CH<sub>4</sub>:ME and CH<sub>4</sub>:OM (in steers) and CH<sub>4</sub>:SCFA (in sheep), while the interaction was not significant (P > 0.05) for CH<sub>4</sub>:SCFA (in steers) and CH<sub>4</sub>:ME and CH<sub>4</sub>:OM (in sheep). In conclusion, the interaction of M. oleifera seeds and probiotics in the feeding diet reduced GHG emissions and affected the fermentation profile of steers and sheep.</p>\",\"PeriodicalId\":7537,\"journal\":{\"name\":\"AMB Express\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-07-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11289196/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"AMB Express\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1186/s13568-024-01744-x\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"AMB Express","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1186/s13568-024-01744-x","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Deciphering the role of Moringa oleifera seeds and probiotic bacteria on mitigation of biogas production from ruminants.
Maintaining cleaner and more sustainable ecosystems by mitigating greenhouse gas (GHG) emissions from livestock through dietary manipulation is in demand. This study was aimed to assess the effect of Moringa oleifera seeds and probiotics (Pediococcus acidilactici BX-B122 and Bacillus coagulans BX-B118) as feed supplements on GHG production and fermentation profile from steers and sheep. The treatments included diets containing 0, 6, 12, and 18% of M. oleifera seeds meal and a mixture of probiotic bacteria (0.2 ml/g of diet). Total biogas production, CH4, CO, and H2S emission from animals (up to 48 h), rumen fermentation profile, and CH4 conversion efficiency were recorded using standard protocols. Results showed interaction among M. oleifera seeds and probiotics on asymptotic biogas production and total biogas production up to 48 h (P < 0.05). The rate of CH4 emission in steers was reduced from 0.1694 to 0.0447 ml/h using 6 and 18% of M. oleifera seeds (P < 0.05). Asymptotic CO and the rate of CO production were increased (P < 0.05) by supplementing different doses of M. oleifera seeds and probiotics. Adding 12% of M. oleifera seeds and probiotics reduced H2S production from 0.0675 to 0.0112 ml H2S/g DM (at 48 h of fermentation) in steers. In sheep, the additives mitigated H2S production from 0.0364 to 0.0029 ml H2S/g DM (at 48 h of fermentation), however there were not interaction (P = 0.7744). In addition, M. oleifera seeds and probiotics reduced the pH level and dry matter degradability (DMD) in steers and sheep (P < 0.0001) showing a positive impact on CH4:ME and CH4:OM (in steers) and CH4:SCFA (in sheep), while the interaction was not significant (P > 0.05) for CH4:SCFA (in steers) and CH4:ME and CH4:OM (in sheep). In conclusion, the interaction of M. oleifera seeds and probiotics in the feeding diet reduced GHG emissions and affected the fermentation profile of steers and sheep.
期刊介绍:
AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.