D.V.S. Priyanka, Subha Das, K. Raju, Princy S. Soman, Nikhil Chaudhary
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The cytomorphologies of both the smears were compared based on five parameters, clarity of staining, uniformity of staining, overall morphology, nuclear details, and cytoplasmic details, and the final score was calculated by adding all the scores: Total Score ≤2, poor score; 3–4, good score; 5, excellent score.\n \n \n \n Cervical smears fixed in honey showed similar features to ethanol-fixed smears. Fluid smears fixed in honey and stained with PAP yielded superior results compared to those stained with H and E, as shown by a kappa value of 0.259. However, neither of the smears demonstrated statistical significance. The FNAC samples stained with H and E and PAP exhibited nearly identical results, with a kappa value of 0.688 and 0.647, respectively. FNAC and PAP smears were the next two most successful cytological smear types, with cervical smears exhibiting the best results of the three.\n \n \n \n In honey-fixed smears, we can easily observe all cellular features with clarity. 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To study cytomorphological features of smears fixed in 20% unprocessed honey. 2. To study the efficacy of honey as a fixative when compared with ethanol in both hematoxylin and eosin (H and E) stain and Papanicolaou (PAP) stain for cytological samples.\\n \\n \\n \\n A cross-sectional study was done in the Department of Pathology for 2 months on 100 cytological samples consisting of body fluids (peritoneal and pleural), cervical smears, and FNACs. These smears were fixed separately in 95% ethanol and 20% unprocessed honey for 5 minutes and were stained with both PAP and H and E stain. The cytomorphologies of both the smears were compared based on five parameters, clarity of staining, uniformity of staining, overall morphology, nuclear details, and cytoplasmic details, and the final score was calculated by adding all the scores: Total Score ≤2, poor score; 3–4, good score; 5, excellent score.\\n \\n \\n \\n Cervical smears fixed in honey showed similar features to ethanol-fixed smears. 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引用次数: 0
摘要
固定在固定细胞方面起着重要作用,可使细胞便于评估。乙醇(95%)被用作标准的细胞学固定剂。蜂蜜可作为一种天然固定剂。 1.研究用 20% 未加工蜂蜜固定的涂片的细胞形态特征。2.与乙醇相比,研究蜂蜜作为细胞学样本苏木精和伊红(H 和 E)染色及巴氏(PAP)染色固定剂的功效。 病理科对 100 份细胞学样本(包括体液(腹膜和胸膜)、宫颈涂片和 FNAC)进行了为期 2 个月的横断面研究。这些涂片分别在 95% 的乙醇和 20% 的未加工蜂蜜中固定 5 分钟,并用 PAP 和 H、E 染色法染色。根据染色的清晰度、染色的均匀性、整体形态、核细节和细胞质细节这五个参数对两种涂片的细胞形态进行比较,并将所有得分相加计算出最终得分:总分≤2分,差;3-4分,好;5分,优。 用蜂蜜固定的宫颈涂片与用乙醇固定的涂片显示出相似的特征。与用 H 和 E 染色的涂片相比,用蜂蜜固定并用 PAP 染色的液体涂片结果更佳,kappa 值为 0.259。不过,这两种涂片都没有统计学意义。用 H 和 E 及 PAP 染色的 FNAC 涂片结果几乎相同,卡帕值分别为 0.688 和 0.647。FNAC 涂片和 PAP 涂片是随后两种最成功的细胞学涂片类型,而宫颈涂片的结果是三种涂片中最好的。 在蜜固定涂片中,我们很容易清晰地观察到所有细胞特征。它提高了安全性,改善了实验室的工作环境。在资源有限的情况下,蜂蜜可作为一种有效的替代品。
Comparative Evaluation of Honey Versus Alcohol as a Fixative in Cytological Samples: An Institutional Study
Fixation plays an important role in fixing the cells, making them amenable for evaluation. Ethanol (95%) has been used as a standard cytological fixative. Honey can be introduced as a natural fixative.
1. To study cytomorphological features of smears fixed in 20% unprocessed honey. 2. To study the efficacy of honey as a fixative when compared with ethanol in both hematoxylin and eosin (H and E) stain and Papanicolaou (PAP) stain for cytological samples.
A cross-sectional study was done in the Department of Pathology for 2 months on 100 cytological samples consisting of body fluids (peritoneal and pleural), cervical smears, and FNACs. These smears were fixed separately in 95% ethanol and 20% unprocessed honey for 5 minutes and were stained with both PAP and H and E stain. The cytomorphologies of both the smears were compared based on five parameters, clarity of staining, uniformity of staining, overall morphology, nuclear details, and cytoplasmic details, and the final score was calculated by adding all the scores: Total Score ≤2, poor score; 3–4, good score; 5, excellent score.
Cervical smears fixed in honey showed similar features to ethanol-fixed smears. Fluid smears fixed in honey and stained with PAP yielded superior results compared to those stained with H and E, as shown by a kappa value of 0.259. However, neither of the smears demonstrated statistical significance. The FNAC samples stained with H and E and PAP exhibited nearly identical results, with a kappa value of 0.688 and 0.647, respectively. FNAC and PAP smears were the next two most successful cytological smear types, with cervical smears exhibiting the best results of the three.
In honey-fixed smears, we can easily observe all cellular features with clarity. It enhances safety and the working environment in the laboratory. Honey can serve as an effective substitute in situations where resources are limited.
期刊介绍:
The Journal of Cytology is the official Quarterly publication of the Indian Academy of Cytologists. It is in the 25th year of publication in the year 2008. The journal covers all aspects of diagnostic cytology, including fine needle aspiration cytology, gynecological and non-gynecological cytology. Articles on ancillary techniques, like cytochemistry, immunocytochemistry, electron microscopy, molecular cytopathology, as applied to cytological material are also welcome. The journal gives preference to clinically oriented studies over experimental and animal studies. The Journal would publish peer-reviewed original research papers, case reports, systematic reviews, meta-analysis, and debates.