Seok-Hui Lee , Eun-Ju Kim , Seo-Young Ju , Yong Li , Sei-Jung Lee
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引用次数: 0
摘要
Momordica cochinchinensis(MC),俗称嘎子果,是一种富含抗氧化剂和生物活性化合物的热带水果。本研究旨在阐明 MC 对直径小于 10 μm 的细颗粒物(< PM10)诱导表皮角质细胞 HaCaT 细胞凋亡的影响。我们发现,PM10 通过细胞毒性机制大大降低了 HaCaT 细胞的活力。然而,用浓度为 10 μg/mL 的 MC 处理后,被 PM10 削弱的细胞活力明显恢复。MC主要通过阻止活性氧(ROS)的生成,减少了细胞外信号调节激酶(ERK)和p38丝裂原活化蛋白激酶(MAPK)对受PM10作用的HaCaT细胞的激活。此外,MC 还通过抑制转录因子核因子-卡巴 B(NF-κB)的活化,对 B 细胞淋巴瘤 2(Bcl-2)、Bcl-2 相关 X 蛋白(Bax)和裂解的 Caspase-3 等与细胞凋亡有关的基因的表达具有调节作用。这些研究结果表明,MC 有助于中和由 PM10 等环境污染物产生的自由基的凋亡信号通路,这些自由基有可能损伤皮肤细胞并加速衰老过程。
Momordica cochinchinensis extract alleviates oxidative stress and skin damage caused by fine particulate matter
Momordica cochinchinensis (MC), commonly known as gac fruit, is a tropical fruit rich in antioxidants and bioactive compounds. This research aimed to elucidate the effect of MC on apoptosis induced by fine particulate matter with a diameter of less than 10 μm (< PM10) in epidermal keratinocyte HaCaT cells. We found that PM10 significantly diminish the viability of HaCaT cells through cytotoxic mechanisms. However, the treatment with MC at a concentration of 10 μg/mL notably restored the cellular viability decreased by PM10. MC reduced the activation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) by mainly preventing the generation of reactive oxygen species (ROS) in HaCaT cells subjected to PM10. Furthermore, MC exhibited a regulatory effect on the expression of genes associated with apoptosis, including B-Cell Lymphoma 2 (Bcl-2), Bcl-2 associated X protein (Bax), and cleaved caspase-3 by inhibiting the activation of the transcription factor nuclear factor-kappa B (NF-κB). These findings demonstrate that MC aids in neutralizing the apoptotic signaling pathway of free radicals produced by environmental pollutants such as PM10, which have the potential to damage skin cells and accelerate the aging process.
期刊介绍:
Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed.
Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.