{"title":"硅藻 Phaeodactylum tricornutum 在氮饥饿和恢复过程中的中性脂质和菊粉代谢途径","authors":"","doi":"10.1016/j.algal.2024.103621","DOIUrl":null,"url":null,"abstract":"<div><p>Oleaginous diatoms, including the marine model species <em>Phaeodactylum tricornutum</em>, have been identified as promising sources of compounds with a biotechnological interest including triacylglycerol and the soluble polysaccharide chrysolaminarin. These two molecules are accumulated in cells under nutrient starvation conditions, however, their consumption after nutrient replenishment lack a precise characterization. In this study, two ecotypes of <em>P. tricornutum</em> (Pt1 and Pt4) have been subjected to a nitrate starvation followed by a resupply to monitor their response through biochemical assays and gene expression quantification. We highlighted that both ecotypes experienced a two-step response to nitrogen resupply, with a rapid initial consumption of stored soluble carbohydrates probably allowing the restart of photosynthesis and protein synthesis, followed by a consumption of neutral lipids fueling cell division. Some genes were particularly upregulated after resupply, especially those encoding the lipases Phatr3_EG02408 and Phatr3_EG00720 and the exoglucosidase Phatr3_J43302, suggesting their role in degradation processes. Additionally, ecotype Pt4 recovered faster from stress than ecotype Pt1, possibly due to differences in specific gene regulations. Overall, these findings provide potential targets for understanding lipid and carbohydrate degradation, and for creating high-lipid producing strains.</p></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2211926424002339/pdfft?md5=aa860c5089647848ec528a2ca8cd6897&pid=1-s2.0-S2211926424002339-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Neutral lipid and chrysolaminarin metabolic pathways during nitrogen starvation and recovery in the diatom Phaeodactylum tricornutum\",\"authors\":\"\",\"doi\":\"10.1016/j.algal.2024.103621\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Oleaginous diatoms, including the marine model species <em>Phaeodactylum tricornutum</em>, have been identified as promising sources of compounds with a biotechnological interest including triacylglycerol and the soluble polysaccharide chrysolaminarin. These two molecules are accumulated in cells under nutrient starvation conditions, however, their consumption after nutrient replenishment lack a precise characterization. In this study, two ecotypes of <em>P. tricornutum</em> (Pt1 and Pt4) have been subjected to a nitrate starvation followed by a resupply to monitor their response through biochemical assays and gene expression quantification. We highlighted that both ecotypes experienced a two-step response to nitrogen resupply, with a rapid initial consumption of stored soluble carbohydrates probably allowing the restart of photosynthesis and protein synthesis, followed by a consumption of neutral lipids fueling cell division. Some genes were particularly upregulated after resupply, especially those encoding the lipases Phatr3_EG02408 and Phatr3_EG00720 and the exoglucosidase Phatr3_J43302, suggesting their role in degradation processes. Additionally, ecotype Pt4 recovered faster from stress than ecotype Pt1, possibly due to differences in specific gene regulations. Overall, these findings provide potential targets for understanding lipid and carbohydrate degradation, and for creating high-lipid producing strains.</p></div>\",\"PeriodicalId\":7855,\"journal\":{\"name\":\"Algal Research-Biomass Biofuels and Bioproducts\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2211926424002339/pdfft?md5=aa860c5089647848ec528a2ca8cd6897&pid=1-s2.0-S2211926424002339-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Algal Research-Biomass Biofuels and Bioproducts\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2211926424002339\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Algal Research-Biomass Biofuels and Bioproducts","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2211926424002339","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Neutral lipid and chrysolaminarin metabolic pathways during nitrogen starvation and recovery in the diatom Phaeodactylum tricornutum
Oleaginous diatoms, including the marine model species Phaeodactylum tricornutum, have been identified as promising sources of compounds with a biotechnological interest including triacylglycerol and the soluble polysaccharide chrysolaminarin. These two molecules are accumulated in cells under nutrient starvation conditions, however, their consumption after nutrient replenishment lack a precise characterization. In this study, two ecotypes of P. tricornutum (Pt1 and Pt4) have been subjected to a nitrate starvation followed by a resupply to monitor their response through biochemical assays and gene expression quantification. We highlighted that both ecotypes experienced a two-step response to nitrogen resupply, with a rapid initial consumption of stored soluble carbohydrates probably allowing the restart of photosynthesis and protein synthesis, followed by a consumption of neutral lipids fueling cell division. Some genes were particularly upregulated after resupply, especially those encoding the lipases Phatr3_EG02408 and Phatr3_EG00720 and the exoglucosidase Phatr3_J43302, suggesting their role in degradation processes. Additionally, ecotype Pt4 recovered faster from stress than ecotype Pt1, possibly due to differences in specific gene regulations. Overall, these findings provide potential targets for understanding lipid and carbohydrate degradation, and for creating high-lipid producing strains.
期刊介绍:
Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment