Yang Liu , Wanbing Pan , Lingyu Li , Jianquan Gong , Hongjing Dong , Bin Zhang , Jiahao Lin , Zhenjia Zheng
{"title":"牛蒡多糖-锌（II）复合物的制备、表征及其免疫活性评估","authors":"Yang Liu , Wanbing Pan , Lingyu Li , Jianquan Gong , Hongjing Dong , Bin Zhang , Jiahao Lin , Zhenjia Zheng","doi":"10.1016/j.focha.2024.100784","DOIUrl":null,"url":null,"abstract":"<div><p>A novel <em>Arctium lappa</em> L. polysaccharide zinc (ALP-Zn) complex was synthesized with success. The structural characteristics, stability, and <em>in vitro</em> digestive properties of ALP-Zn were studied. Moreover, the immunoreactivity of ALP-Zn on murine bone marrow-derived dendritic cells (BMDCs) was investigated by examining surface molecule expression, cytokine production and endocytosis. The results showed that the weight-average molecular weight (Mw) of ALP-Zn was 60,268 Da. ALP-Zn mainly consisted of fructose, arabinose, galactose, and glucose in a mass ratio of 50.74:27.94:12.49:7.26. Zn-O characteristic peak was identified by fourier transform infrared spectroscopy at 433.42 cm<sup>−1</sup>, indicating that zinc was successfully complexed with ALP. The stability and <em>in vitro</em> digestive properties suggested that ALP-Zn had good stability and biological acceptability. Furthermore, ALP and ALP-Zn demonstrated excellent immunological effects on dendritic cells (DCs). After being treated with ALP and ALP-Zn, CD80 and CD86 expression on the surface of DCs were increased and DCs maturation was induced. Moreover, ALP and ALP-Zn also significantly reduced DC endocytosis and increased cytokine (IFN-<em>γ</em>, TNF-<em>α</em>, IL-1<em>β</em> and IL-6). As a consequence, ALP-Zn complex could be as a potential zinc supplement with immune properties.</p></div>","PeriodicalId":73040,"journal":{"name":"Food chemistry advances","volume":"5 ","pages":"Article 100784"},"PeriodicalIF":0.0000,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772753X24001801/pdfft?md5=0582604a64efeeeca9866ec284834288&pid=1-s2.0-S2772753X24001801-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activity\",\"authors\":\"Yang Liu , Wanbing Pan , Lingyu Li , Jianquan Gong , Hongjing Dong , Bin Zhang , Jiahao Lin , Zhenjia Zheng\",\"doi\":\"10.1016/j.focha.2024.100784\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A novel <em>Arctium lappa</em> L. polysaccharide zinc (ALP-Zn) complex was synthesized with success. The structural characteristics, stability, and <em>in vitro</em> digestive properties of ALP-Zn were studied. Moreover, the immunoreactivity of ALP-Zn on murine bone marrow-derived dendritic cells (BMDCs) was investigated by examining surface molecule expression, cytokine production and endocytosis. The results showed that the weight-average molecular weight (Mw) of ALP-Zn was 60,268 Da. ALP-Zn mainly consisted of fructose, arabinose, galactose, and glucose in a mass ratio of 50.74:27.94:12.49:7.26. Zn-O characteristic peak was identified by fourier transform infrared spectroscopy at 433.42 cm<sup>−1</sup>, indicating that zinc was successfully complexed with ALP. The stability and <em>in vitro</em> digestive properties suggested that ALP-Zn had good stability and biological acceptability. Furthermore, ALP and ALP-Zn demonstrated excellent immunological effects on dendritic cells (DCs). After being treated with ALP and ALP-Zn, CD80 and CD86 expression on the surface of DCs were increased and DCs maturation was induced. Moreover, ALP and ALP-Zn also significantly reduced DC endocytosis and increased cytokine (IFN-<em>γ</em>, TNF-<em>α</em>, IL-1<em>β</em> and IL-6). As a consequence, ALP-Zn complex could be as a potential zinc supplement with immune properties.</p></div>\",\"PeriodicalId\":73040,\"journal\":{\"name\":\"Food chemistry advances\",\"volume\":\"5 \",\"pages\":\"Article 100784\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2772753X24001801/pdfft?md5=0582604a64efeeeca9866ec284834288&pid=1-s2.0-S2772753X24001801-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food chemistry advances\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2772753X24001801\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food chemistry advances","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772753X24001801","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activity
A novel Arctium lappa L. polysaccharide zinc (ALP-Zn) complex was synthesized with success. The structural characteristics, stability, and in vitro digestive properties of ALP-Zn were studied. Moreover, the immunoreactivity of ALP-Zn on murine bone marrow-derived dendritic cells (BMDCs) was investigated by examining surface molecule expression, cytokine production and endocytosis. The results showed that the weight-average molecular weight (Mw) of ALP-Zn was 60,268 Da. ALP-Zn mainly consisted of fructose, arabinose, galactose, and glucose in a mass ratio of 50.74:27.94:12.49:7.26. Zn-O characteristic peak was identified by fourier transform infrared spectroscopy at 433.42 cm−1, indicating that zinc was successfully complexed with ALP. The stability and in vitro digestive properties suggested that ALP-Zn had good stability and biological acceptability. Furthermore, ALP and ALP-Zn demonstrated excellent immunological effects on dendritic cells (DCs). After being treated with ALP and ALP-Zn, CD80 and CD86 expression on the surface of DCs were increased and DCs maturation was induced. Moreover, ALP and ALP-Zn also significantly reduced DC endocytosis and increased cytokine (IFN-γ, TNF-α, IL-1β and IL-6). As a consequence, ALP-Zn complex could be as a potential zinc supplement with immune properties.
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