{"title":"Pestalotiopsis kenyana 在中国杜鹃花上引发叶斑病","authors":"","doi":"10.1016/j.cropro.2024.106859","DOIUrl":null,"url":null,"abstract":"<div><p><em>Rhododendron agastum</em>, a well-known flower with significant aesthetic and medicinal value, belongs to the <em>Rhododendron</em> genus in the Ericaceae family. In April 2023, a leaf spot disease on <em>R. agastum</em> plants was found in the Baili Azalea Forest Area in Guizhou province, China. Approximately 400 plants were surveyed, and the incidence of leaf spot on <em>R. agastum</em> leaves was 20%. The damaged leaves had gray-white irregular diseased spots with black-brown margins and black conidiomata on the surface of the spots. When the disease is severe, the leaves fall. Leaf spot disease is a serious problem for <em>rhododendrons</em>, reducing their ornamental value and causing significant economic losses. To identify the pathogen, we conducted pathogen isolation and pathogenicity study. The pathogen was identified as <em>Pestalotiopsis kenyana</em> by morphological features and combined analysis of multiple genes (<em>ITS</em>, <em>tef1-α</em>, and <em>Tub2</em>). Koch's postulates was tested by isolating and reidentifying the pathogen from inoculated plants, showing that <em>P</em>. <em>kenyana</em> was the causative agent of the disease. To our knowledge, this is the first report of <em>P. kenyana</em> causing leaf spot in <em>R. agastum</em>. This study gives important information for the identification and control of the leaf spot disease caused by <em>P</em>. <em>kenyana</em>.</p></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pestalotiopsis kenyana causes leaf spot disease on Rhododendron agastum in China\",\"authors\":\"\",\"doi\":\"10.1016/j.cropro.2024.106859\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Rhododendron agastum</em>, a well-known flower with significant aesthetic and medicinal value, belongs to the <em>Rhododendron</em> genus in the Ericaceae family. In April 2023, a leaf spot disease on <em>R. agastum</em> plants was found in the Baili Azalea Forest Area in Guizhou province, China. Approximately 400 plants were surveyed, and the incidence of leaf spot on <em>R. agastum</em> leaves was 20%. The damaged leaves had gray-white irregular diseased spots with black-brown margins and black conidiomata on the surface of the spots. When the disease is severe, the leaves fall. Leaf spot disease is a serious problem for <em>rhododendrons</em>, reducing their ornamental value and causing significant economic losses. To identify the pathogen, we conducted pathogen isolation and pathogenicity study. The pathogen was identified as <em>Pestalotiopsis kenyana</em> by morphological features and combined analysis of multiple genes (<em>ITS</em>, <em>tef1-α</em>, and <em>Tub2</em>). Koch's postulates was tested by isolating and reidentifying the pathogen from inoculated plants, showing that <em>P</em>. <em>kenyana</em> was the causative agent of the disease. To our knowledge, this is the first report of <em>P. kenyana</em> causing leaf spot in <em>R. agastum</em>. This study gives important information for the identification and control of the leaf spot disease caused by <em>P</em>. <em>kenyana</em>.</p></div>\",\"PeriodicalId\":10785,\"journal\":{\"name\":\"Crop Protection\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-07-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Crop Protection\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0261219424002874\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Protection","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0261219424002874","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
摘要
杜鹃花(Rhododendron agastum)属杜鹃花科杜鹃花属,是一种著名的花卉,具有重要的观赏价值和药用价值。2023 年 4 月,中国贵州省百里杜鹃林区发现了杜鹃花叶斑病。经调查,约有 400 株杜鹃叶片发生叶斑病,发病率为 20%。被害叶片上有灰白色不规则病斑,边缘黑褐色,病斑表面有黑色分生孢子器。病害严重时,叶片会掉落。叶斑病是杜鹃花的一个严重问题,它降低了杜鹃花的观赏价值,并造成重大经济损失。为了确定病原体,我们进行了病原体分离和致病性研究。通过形态特征和多个基因(ITS、tef1-α 和 Tub2)的综合分析,确定病原体为 Pestalotiopsis kenyana。通过从接种植物中分离并重新鉴定病原体,检验了科赫推论,结果表明 P. kenyana 是该病的病原体。据我们所知,这是首次报道 P. kenyana 在姬松茸中引起叶斑病。这项研究为鉴定和控制 P. kenyana 引起的叶斑病提供了重要信息。
Pestalotiopsis kenyana causes leaf spot disease on Rhododendron agastum in China
Rhododendron agastum, a well-known flower with significant aesthetic and medicinal value, belongs to the Rhododendron genus in the Ericaceae family. In April 2023, a leaf spot disease on R. agastum plants was found in the Baili Azalea Forest Area in Guizhou province, China. Approximately 400 plants were surveyed, and the incidence of leaf spot on R. agastum leaves was 20%. The damaged leaves had gray-white irregular diseased spots with black-brown margins and black conidiomata on the surface of the spots. When the disease is severe, the leaves fall. Leaf spot disease is a serious problem for rhododendrons, reducing their ornamental value and causing significant economic losses. To identify the pathogen, we conducted pathogen isolation and pathogenicity study. The pathogen was identified as Pestalotiopsis kenyana by morphological features and combined analysis of multiple genes (ITS, tef1-α, and Tub2). Koch's postulates was tested by isolating and reidentifying the pathogen from inoculated plants, showing that P. kenyana was the causative agent of the disease. To our knowledge, this is the first report of P. kenyana causing leaf spot in R. agastum. This study gives important information for the identification and control of the leaf spot disease caused by P. kenyana.
期刊介绍:
The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics:
-Abiotic damage-
Agronomic control methods-
Assessment of pest and disease damage-
Molecular methods for the detection and assessment of pests and diseases-
Biological control-
Biorational pesticides-
Control of animal pests of world crops-
Control of diseases of crop plants caused by microorganisms-
Control of weeds and integrated management-
Economic considerations-
Effects of plant growth regulators-
Environmental benefits of reduced pesticide use-
Environmental effects of pesticides-
Epidemiology of pests and diseases in relation to control-
GM Crops, and genetic engineering applications-
Importance and control of postharvest crop losses-
Integrated control-
Interrelationships and compatibility among different control strategies-
Invasive species as they relate to implications for crop protection-
Pesticide application methods-
Pest management-
Phytobiomes for pest and disease control-
Resistance management-
Sampling and monitoring schemes for diseases, nematodes, pests and weeds.