雄性大鼠脑缺血再灌注期间服用褪黑素和 N-乙酰半胱氨酸可减少氧化应激损伤

IF 2 Q3 NEUROSCIENCES
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引用次数: 0

摘要

大脑中动脉闭塞症(MCAO)是诱发啮齿类动物缺血性中风的一种模型,可导致破坏性脑损伤。氧化应激(OS)在缺血发病机制中起着至关重要的作用。本研究探讨了褪黑素和 N-乙酰半胱氨酸对缺血再灌注诱导的雄性大鼠大脑皮层氧化应激损伤的影响。30 只雄性 Wistar 大鼠被分为假组、缺血组、NAC 组、褪黑素组和 NAC + 褪黑素组。除假组外,其他各组均接受左侧 MCAO,治疗组在 24 和 48 小时后腹腔注射 50 毫克/千克 N-乙酰半胱氨酸(NAC)或 5 毫克/千克褪黑素或两者的组合。术后 24 小时和 72 小时,检查动物的感觉和运动活动。牺牲大鼠后解剖大脑皮层,估算梗死体积,并通过酶联免疫吸附试验(ELISA)分析谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)和核因子红细胞-2相关因子2(Nrf2)的浓度。结果表明,与缺血组相比,NAC + 褪黑素组的感觉运动活性升高,梗死体积率降低(p≤ 0.05)。与缺血组相比,NAC + 褪黑激素组的 SOD 浓度显著增加,MDA 显著降低(p≤ 0.05)。因此可以得出结论,同时服用 NAC 和褪黑素可以通过调节 SOD 和 MDA 减少脑梗死体积,改善神经功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Reduced ischemia‐reperfusion oxidative stress injury by melatonin and N‐acetylcysteine in the male rat brain

Middle cerebral artery occlusion (MCAO) is a model for inducing ischemic stroke in rodents, leading to devastating brain damage. Oxidative stress (OS) plays a crucial role in the pathogenesis of ischemia. In this study, the effect of melatonin and N-acetylcysteine on ischemia-reperfusion-induced oxidative stress injury in the cerebral cortex of male rats was investigated. 30 male Wistar rats were divided into sham, ischemic, NAC, melatonin and NAC + melatonin groups. All groups, except the sham group, underwent MCAO on the left side, and the treatment groups received intraperitoneal injections of either 50 mg/kg N-acetylcysteine (NAC) or 5 mg/kg melatonin or a combination of both 24 and 48 hours later. At 24 and 72 hours after surgery, the animals were examined for sensory and motor activity. The cerebral cortex was dissected after sacrificing the rats, infarct volume estimated and the concentrations of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and nuclear factor erythroid-2 related factor 2 (Nrf2) were analyzed by enzyme-linked immunosorbent assay (ELISA). The results indicate that the NAC + melatonin group exhibited elevated sensory-motor activity and a reduced infarct volume rate in comparison to the ischemic group (p≤ 0.05). Compared to the ischemic group, the NAC + melatonin group showed a significant increase in SOD concentration and a significant decrease in MDA (p≤ 0.05). It can therefore be concluded that the simultaneous administration of NAC and melatonin can reduce the cerebral infarction volume, and improve neurological functions by modulating SOD and MDA.

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来源期刊
IBRO Neuroscience Reports
IBRO Neuroscience Reports Neuroscience-Neuroscience (all)
CiteScore
2.80
自引率
0.00%
发文量
99
审稿时长
14 weeks
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