基于 T7 等温扩增结合聚集诱导发光策略的超灵敏 HCV 检测荧光传感平台

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Wuxiu Guo , Xin Zhu , Jinchao Li , Linhai Li
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引用次数: 0

摘要

丙型肝炎病毒感染是一个重大的全球公共卫生问题。荧光技术(FL)是一种前景广阔的生物传感器,然而,许多传统染料在高浓度时荧光减弱或无发射。为了突破这一桎梏,我们利用基于 FLRNAs 的 T7 等温转录扩增 RNA 诊断结合 AIE 发光剂(FTAR)策略,开发了一种超灵敏的基于 AIE(聚集诱导发射)的荧光传感平台,用于检测 HCV。在 FTAR 策略中,T7 转录扩增过程负责识别目标 HCV 并产生 Pepper aptamer,后者可与 AIE 源 HBC 形成纳米颗粒聚集体,产生高强度 FL。多集成信号放大策略带来了超灵敏度。此外,在最佳实验条件下,HCV 的检测范围可达 100aM-100pM。所提出的策略已成功应用于检测临床样本中的 HCV。总之,这项研究首次利用 AIEgens 内旋转的适配体限制来实现聚集诱导发射,并成功开发了用于 HCV 诊断的特异、灵敏和快速的 FTAR 检测方法。所提出的 FTAR 平台操作简便、分析时间短、成本低、定量能力强,可为床旁检测(POCT)提供一种前景广阔的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

An ultrasensitive fluorescence sensing platform for HCV detection based on the T7 isothermal amplification combined with aggregation-induced emission luminogens strategy

An ultrasensitive fluorescence sensing platform for HCV detection based on the T7 isothermal amplification combined with aggregation-induced emission luminogens strategy

Hepatitis C virus infection constitutes a significant global public health concern. Fluorescence technique (FL) is a promising biosensor, however, many conventional dyes exhibit reduced fluorescence or no emission at high concentrations. To break through this shackle, an ultrasensitive AIE-based (aggregation-induced emission) FL sensing platform was developed for HCV detection by using the FLRNAs-based T7 isothermal transcription amplification RNA diagnosis combined with AIE luminogens (FTAR) strategy. In the FTAR strategy, the T7 transcription amplification process was responsible for the recognition of the target HCV and produced Pepper aptamer, which could form aggregates of nanoparticles with the HBC, an AIEgen, to produce high-intensity FL. The multi-integrated signal amplification strategy led to ultrasensitivity. Moreover, under the optimal experimental conditions, HCV could be assayed in the range of 100aM-100pM. The proposed strategy has been successfully applied in detecting HCV in clinical samples. In summary, this research was the first to utilize aptamer restriction of AIEgens internal rotation to achieve aggregation-induced emission and presented the successful development of a specific, sensitive and rapid FTAR assay for HCV diagnosis. The proposed FTAR platform with facile operation, short analysis time, low-cost as well as excellent quantification ability could provide a promising tool for point-of-care testing (POCT).

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来源期刊
CiteScore
9.60
自引率
0.00%
发文量
60
审稿时长
49 days
期刊介绍: Sensors and Actuators Reports is a peer-reviewed open access journal launched out from the Sensors and Actuators journal family. Sensors and Actuators Reports is dedicated to publishing new and original works in the field of all type of sensors and actuators, including bio-, chemical-, physical-, and nano- sensors and actuators, which demonstrates significant progress beyond the current state of the art. The journal regularly publishes original research papers, reviews, and short communications. For research papers and short communications, the journal aims to publish the new and original work supported by experimental results and as such purely theoretical works are not accepted.
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