Azamat Temerdashev , Pavel N. Nesterenko , Sanka N. Atapattu , Yu-Qi Feng , Maria Zorina , Kseniya Zhurkina , Elina Gashimova , Maxim O. Steshin , Victor V. Dotsenko
{"title":"利用超高效液相色谱-高分辨质谱法测定尿氨基酸时作为衍生试剂的邻苯二甲酰甘酰氯","authors":"Azamat Temerdashev , Pavel N. Nesterenko , Sanka N. Atapattu , Yu-Qi Feng , Maria Zorina , Kseniya Zhurkina , Elina Gashimova , Maxim O. Steshin , Victor V. Dotsenko","doi":"10.1016/j.jcoa.2024.100162","DOIUrl":null,"url":null,"abstract":"<div><p>A new derivatization reagent, phthalylglycyl chloride (<strong>PG-Cl</strong>), for the analysis of urinary amino acids was demonstrated using reversed-phase ultra-high performance liquid chromatography coupled to electrospray ionization-quadrupole-time-of-flight mass spectrometer. The study compares the limits of detection and quantification for different sample preparation techniques with the commonly used and effective method of derivatization using dansyl chloride. The stability of the derivatives was investigated, and the study includes an example of urinary amino acid analysis using PG-Cl derivatization. The optimal values for pH, temperature, and concentration of the derivatization reagent were established. Limits of quantification in the range of 0.5–500 µg/mL were obtained for different amino acids. The possibility of phthalylglycyl chloride usage for non-target screening applications and targeted analysis of amino acids in urine was discussed. An advantages and issues of PG-Cl in comparison with commonly used acyl-chlorides (FMOC-Cl and DNS-Cl) provided and discussed.</p></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"6 ","pages":"Article 100162"},"PeriodicalIF":0.0000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772391724000495/pdfft?md5=6691640ed40d6a22a0605c3ed55bb40a&pid=1-s2.0-S2772391724000495-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry\",\"authors\":\"Azamat Temerdashev , Pavel N. Nesterenko , Sanka N. Atapattu , Yu-Qi Feng , Maria Zorina , Kseniya Zhurkina , Elina Gashimova , Maxim O. Steshin , Victor V. Dotsenko\",\"doi\":\"10.1016/j.jcoa.2024.100162\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A new derivatization reagent, phthalylglycyl chloride (<strong>PG-Cl</strong>), for the analysis of urinary amino acids was demonstrated using reversed-phase ultra-high performance liquid chromatography coupled to electrospray ionization-quadrupole-time-of-flight mass spectrometer. The study compares the limits of detection and quantification for different sample preparation techniques with the commonly used and effective method of derivatization using dansyl chloride. The stability of the derivatives was investigated, and the study includes an example of urinary amino acid analysis using PG-Cl derivatization. The optimal values for pH, temperature, and concentration of the derivatization reagent were established. Limits of quantification in the range of 0.5–500 µg/mL were obtained for different amino acids. The possibility of phthalylglycyl chloride usage for non-target screening applications and targeted analysis of amino acids in urine was discussed. An advantages and issues of PG-Cl in comparison with commonly used acyl-chlorides (FMOC-Cl and DNS-Cl) provided and discussed.</p></div>\",\"PeriodicalId\":93576,\"journal\":{\"name\":\"Journal of chromatography open\",\"volume\":\"6 \",\"pages\":\"Article 100162\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2772391724000495/pdfft?md5=6691640ed40d6a22a0605c3ed55bb40a&pid=1-s2.0-S2772391724000495-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of chromatography open\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2772391724000495\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of chromatography open","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772391724000495","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry
A new derivatization reagent, phthalylglycyl chloride (PG-Cl), for the analysis of urinary amino acids was demonstrated using reversed-phase ultra-high performance liquid chromatography coupled to electrospray ionization-quadrupole-time-of-flight mass spectrometer. The study compares the limits of detection and quantification for different sample preparation techniques with the commonly used and effective method of derivatization using dansyl chloride. The stability of the derivatives was investigated, and the study includes an example of urinary amino acid analysis using PG-Cl derivatization. The optimal values for pH, temperature, and concentration of the derivatization reagent were established. Limits of quantification in the range of 0.5–500 µg/mL were obtained for different amino acids. The possibility of phthalylglycyl chloride usage for non-target screening applications and targeted analysis of amino acids in urine was discussed. An advantages and issues of PG-Cl in comparison with commonly used acyl-chlorides (FMOC-Cl and DNS-Cl) provided and discussed.