{"title":"雷帕霉素通过雷帕霉素机制靶标(mTOR)信号通路减轻瓦斯爆炸诱发的大鼠脾脏损伤","authors":"","doi":"10.1016/j.bej.2024.109436","DOIUrl":null,"url":null,"abstract":"<div><p>Gas explosion is a recurrent event in coal mining that cause severe spleen damage due to shockwaves, which has no effective treatment. This study aimed to explore the regulatory role of autophagy in gas explosion-induced blast spleen injuries in rats. 120 Sprague-Dawley male rats were randomly divided into 4 groups, including normal control (NC), gas explosion-induced spleen injury (Model, M), autophagy inhibitor 3-methyladenine group (M+3-MA), and induction Rapamycin (RAPA) group (M+RAPA) groups. After explosion, the inhibitor group and induction group rats were immediately given intraperitoneal injection of 3-MA (15 mg/kg)/ RAPA (1 mg/kg). The rats were anesthetized and the spleen were obtained at 24 h, 72 h, and 7 days. The results showed that gas explosion reduced the spleen index, induced spleen blooding, infiltration of inflammatory cells, and increased autophagosomes. The expression of Lc3-Ⅱ was increased, whereas p62 and p-mTOR was decreased significantly (<em>P</em><0.05) in model group. Compared with the model group, RAPA improved the spleen index, spleen bleeding, inflammation, and autophagosomes significantly. The expression of Lc3-Ⅱ was increased, p62 and p-mTOR was decreased significantly, but the opposite results were observed in the inhibitor group. Taken together, we firstly found that RAPA can mitigate gas explosion-induced spleen injury via mTOR signaling, which provides a new idea for the treatment of spleen injury including but not limited to coal mine accidents.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Rapamycin mitigates gas explosion-induced spleen injury in rats via mechanistic target of rapamycin (mTOR) signaling pathway\",\"authors\":\"\",\"doi\":\"10.1016/j.bej.2024.109436\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Gas explosion is a recurrent event in coal mining that cause severe spleen damage due to shockwaves, which has no effective treatment. This study aimed to explore the regulatory role of autophagy in gas explosion-induced blast spleen injuries in rats. 120 Sprague-Dawley male rats were randomly divided into 4 groups, including normal control (NC), gas explosion-induced spleen injury (Model, M), autophagy inhibitor 3-methyladenine group (M+3-MA), and induction Rapamycin (RAPA) group (M+RAPA) groups. After explosion, the inhibitor group and induction group rats were immediately given intraperitoneal injection of 3-MA (15 mg/kg)/ RAPA (1 mg/kg). The rats were anesthetized and the spleen were obtained at 24 h, 72 h, and 7 days. The results showed that gas explosion reduced the spleen index, induced spleen blooding, infiltration of inflammatory cells, and increased autophagosomes. The expression of Lc3-Ⅱ was increased, whereas p62 and p-mTOR was decreased significantly (<em>P</em><0.05) in model group. Compared with the model group, RAPA improved the spleen index, spleen bleeding, inflammation, and autophagosomes significantly. The expression of Lc3-Ⅱ was increased, p62 and p-mTOR was decreased significantly, but the opposite results were observed in the inhibitor group. Taken together, we firstly found that RAPA can mitigate gas explosion-induced spleen injury via mTOR signaling, which provides a new idea for the treatment of spleen injury including but not limited to coal mine accidents.</p></div>\",\"PeriodicalId\":8766,\"journal\":{\"name\":\"Biochemical Engineering Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-07-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemical Engineering Journal\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1369703X24002237\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369703X24002237","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Rapamycin mitigates gas explosion-induced spleen injury in rats via mechanistic target of rapamycin (mTOR) signaling pathway
Gas explosion is a recurrent event in coal mining that cause severe spleen damage due to shockwaves, which has no effective treatment. This study aimed to explore the regulatory role of autophagy in gas explosion-induced blast spleen injuries in rats. 120 Sprague-Dawley male rats were randomly divided into 4 groups, including normal control (NC), gas explosion-induced spleen injury (Model, M), autophagy inhibitor 3-methyladenine group (M+3-MA), and induction Rapamycin (RAPA) group (M+RAPA) groups. After explosion, the inhibitor group and induction group rats were immediately given intraperitoneal injection of 3-MA (15 mg/kg)/ RAPA (1 mg/kg). The rats were anesthetized and the spleen were obtained at 24 h, 72 h, and 7 days. The results showed that gas explosion reduced the spleen index, induced spleen blooding, infiltration of inflammatory cells, and increased autophagosomes. The expression of Lc3-Ⅱ was increased, whereas p62 and p-mTOR was decreased significantly (P<0.05) in model group. Compared with the model group, RAPA improved the spleen index, spleen bleeding, inflammation, and autophagosomes significantly. The expression of Lc3-Ⅱ was increased, p62 and p-mTOR was decreased significantly, but the opposite results were observed in the inhibitor group. Taken together, we firstly found that RAPA can mitigate gas explosion-induced spleen injury via mTOR signaling, which provides a new idea for the treatment of spleen injury including but not limited to coal mine accidents.
期刊介绍:
The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology.
The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields:
Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics
Biosensors and Biodevices including biofabrication and novel fuel cell development
Bioseparations including scale-up and protein refolding/renaturation
Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells
Bioreactor Systems including characterization, optimization and scale-up
Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization
Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals
Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release
Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites
Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation
Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis
Protein Engineering including enzyme engineering and directed evolution.