{"title":"利用超声辅助提取技术优化刺五加茎的抗氧化活性和酚类提取","authors":"S. Ha, J. Jung, Hyeon Cheol Kim, J. Yang","doi":"10.15376/biores.19.3.6325-6338","DOIUrl":null,"url":null,"abstract":"Ultrasound-assisted extraction for bioactive compound retrieval is a viable alternative to traditional extraction methods. Employing ultrasound-assisted extraction, this study aimed to enhance the scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothia-zoline-6-sulfonic acid) (ABTS), total polyphenol content (TPC), and flavonoid content in Ainsliaea acerifolia (A. acerifolia) through response surface methodology (RSM). Initially, the impact of extraction temperature, time, and ethanol concentration on DPPH scavenging capacity, ABTS, TPC, and flavonoid content was assessed. Optimal conditions for maximizing antioxidant activity and TPC were determined as 78% (v/v) ethanol, 60 °C extraction temperature, and 91 min of extraction time. High-performance liquid chromatography (HPLC) analysis of the optimized extract revealed dicaffeoylquinic acid as the primary polyphenol in A. acerifolia extracts, comprising the majority of phenolic compounds (102.06 mg/g DW). This model enabled the optimization of conditions for phenolic compound extraction with antioxidant properties from A. acerifolia, highlighting its potential as a source of antioxidant compounds for industrial, pharmaceutical, and food applications.","PeriodicalId":9172,"journal":{"name":"Bioresources","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimization of antioxidant activity and phenolic extraction from Ainsliaea acerifolia stem using ultrasound-assisted extraction technology\",\"authors\":\"S. Ha, J. Jung, Hyeon Cheol Kim, J. Yang\",\"doi\":\"10.15376/biores.19.3.6325-6338\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Ultrasound-assisted extraction for bioactive compound retrieval is a viable alternative to traditional extraction methods. Employing ultrasound-assisted extraction, this study aimed to enhance the scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothia-zoline-6-sulfonic acid) (ABTS), total polyphenol content (TPC), and flavonoid content in Ainsliaea acerifolia (A. acerifolia) through response surface methodology (RSM). Initially, the impact of extraction temperature, time, and ethanol concentration on DPPH scavenging capacity, ABTS, TPC, and flavonoid content was assessed. Optimal conditions for maximizing antioxidant activity and TPC were determined as 78% (v/v) ethanol, 60 °C extraction temperature, and 91 min of extraction time. High-performance liquid chromatography (HPLC) analysis of the optimized extract revealed dicaffeoylquinic acid as the primary polyphenol in A. acerifolia extracts, comprising the majority of phenolic compounds (102.06 mg/g DW). This model enabled the optimization of conditions for phenolic compound extraction with antioxidant properties from A. acerifolia, highlighting its potential as a source of antioxidant compounds for industrial, pharmaceutical, and food applications.\",\"PeriodicalId\":9172,\"journal\":{\"name\":\"Bioresources\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-07-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioresources\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://doi.org/10.15376/biores.19.3.6325-6338\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, PAPER & WOOD\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresources","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.15376/biores.19.3.6325-6338","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, PAPER & WOOD","Score":null,"Total":0}
引用次数: 0
摘要
超声辅助萃取用于生物活性化合物的提取是传统萃取方法的一种可行替代方法。本研究采用超声辅助萃取技术,通过响应面法(RSM)提高了刺五加(Ainsliaea acerifolia)中2,2-二苯基-1-苦基肼(DPPH)、2,2′-氮杂双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)的清除能力、总多酚含量(TPC)和类黄酮含量。首先评估了提取温度、时间和乙醇浓度对 DPPH 清除能力、ABTS、TPC 和黄酮含量的影响。抗氧化活性和 TPC 最大化的最佳条件被确定为 78%(v/v)乙醇、60 °C 提取温度和 91 分钟提取时间。对优化提取物的高效液相色谱(HPLC)分析表明,二咖啡酰奎宁酸是 A. acerifolia 提取物中的主要多酚,占大部分酚类化合物(102.06 mg/g DW)。该模型优化了从 A. acerifolia 中提取具有抗氧化特性的酚类化合物的条件,凸显了其作为工业、医药和食品应用中抗氧化化合物来源的潜力。
Optimization of antioxidant activity and phenolic extraction from Ainsliaea acerifolia stem using ultrasound-assisted extraction technology
Ultrasound-assisted extraction for bioactive compound retrieval is a viable alternative to traditional extraction methods. Employing ultrasound-assisted extraction, this study aimed to enhance the scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothia-zoline-6-sulfonic acid) (ABTS), total polyphenol content (TPC), and flavonoid content in Ainsliaea acerifolia (A. acerifolia) through response surface methodology (RSM). Initially, the impact of extraction temperature, time, and ethanol concentration on DPPH scavenging capacity, ABTS, TPC, and flavonoid content was assessed. Optimal conditions for maximizing antioxidant activity and TPC were determined as 78% (v/v) ethanol, 60 °C extraction temperature, and 91 min of extraction time. High-performance liquid chromatography (HPLC) analysis of the optimized extract revealed dicaffeoylquinic acid as the primary polyphenol in A. acerifolia extracts, comprising the majority of phenolic compounds (102.06 mg/g DW). This model enabled the optimization of conditions for phenolic compound extraction with antioxidant properties from A. acerifolia, highlighting its potential as a source of antioxidant compounds for industrial, pharmaceutical, and food applications.
期刊介绍:
The purpose of BioResources is to promote scientific discourse and to foster scientific developments related to sustainable manufacture involving lignocellulosic or woody biomass resources, including wood and agricultural residues. BioResources will focus on advances in science and technology. Emphasis will be placed on bioproducts, bioenergy, papermaking technology, wood products, new manufacturing materials, composite structures, and chemicals derived from lignocellulosic biomass.