印度 Chittorgarh 地区具有抗菌潜力的 Digera muricata Mart.

IF 0.7 Q4 MICROBIOLOGY
N. Tomer, Anil Kumar Sharma, M. Ali, Manas Mathur, Hariom Nagar, Ramgopal Dhakar, Amit Sen, Z. Zaidi, Sarmad Moin
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引用次数: 0

摘要

Digera muricata Mart 是一种具有传统治疗特性的植物,属于苋科植物,是一种很有前景的特殊天然产品来源,可用作抗氧化剂、预防剂、抗菌剂、驱虫药、抗糖尿病剂和植物治疗剂。本研究从 Digera muricata Mart 中分离出一种具有生物活性的植物甾醇。分离出的化合物通过 13C、1H NMR、FTIR 和 HRMS 进行了表征。通过抗菌试验和分子对接对分离物进行了表征。发现分离出的植物甾醇(50 µl)对化脓性链球菌的抗菌潜力最大(ZOI-20.0 ± 1.0),其次是无乳链球菌(ZOI-11.3 ± 1.5)、白色念珠菌(ZOI- 09.0 ± 1.0)、肺炎克雷伯菌(ZOI-8.6 ± 1.5)和大肠杆菌(ZOI-8.6 ± 1.5)。分子对接结果表明,植物甾醇在第 32 位和第 58 位与受体 1AI9 结合;在第 76 位与受体 1KZN 结合;在第 46 位(ASN)和第 136 位(ARG)与受体 5L3J 结合;在第 419 位(ARG)和第 582 位(ASP)和第 585 位(ASN)与受体 7WIJ 结合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Novel phytosterol with Antimicrobial Potential of Digera muricata Mart. from Chittorgarh Region, India
Digera muricata Mart, a plant having therapeutic characteristics that has been utilised traditionally, belongs to the Amaranthaceae family, and a promising source of specific natural products utilized as antioxidant, prophylactic, antimicrobial, anthelmintic, anti-diabetic, and allelopathic agent. In the present study, a biologically active phytosterol was isolated from Digera muricata Mart. The isolated compound was characterized by 13C, 1H NMR, FTIR, and HRMS. Characterization of the isolate was done by antimicrobial assay, and molecular docking. The antimicrobial potential of the isolated phytosterol (50 µl) against Streptococcus pyogenes was found to be maximum (ZOI-20.0 ± 1.0), followed by Streptococcus agalactiae (ZOI-11.3±1.5), Candida albicans (ZOI- 09.0 ± 1.0), Klebsiella pneumonia (ZOI-8.6 ± 1.5) and Escherichia coli (ZOI-8.6 ± 1.5). The molecular docking results indicate that the phytosterol binds to the receptor 1AI9 at the 32th and 58th positions; 1KZN receptor at the 76th position, the 5L3J receptor at the 46th (ASN) and 136th (ARG) position; 7WIJ receptor at the 419th (ARG) and 582th (ASP) and 585th (ASN) positions.
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来源期刊
Journal of Pure and Applied Microbiology
Journal of Pure and Applied Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
2.00
自引率
0.00%
发文量
266
审稿时长
11 months
期刊介绍: Journal of Pure and Applied Microbiology (JPAM) is a peer-reviewed, open access international journal of microbiology aims to advance and disseminate research among scientists, academics, clinicians and microbiologists around the world. JPAM publishes high-quality research in all aspects of microbiology in both online and print form on quarterly basis.
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